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Super-resolution acousto-optic modulation confocal imaging device and method

An acousto-optic modulation and imaging device technology, applied in the direction of using optical devices, measuring devices, instruments, etc., can solve problems such as super-resolution acousto-optic modulation imaging methods that have not yet appeared

Active Publication Date: 2013-08-14
HARBIN INST OF TECH
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Problems solved by technology

[0006] So far, there have been no reports on the introduction of super-resolution acousto-optic modulation imaging methods into confocal microscopy systems

Method used

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  • Super-resolution acousto-optic modulation confocal imaging device and method
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  • Super-resolution acousto-optic modulation confocal imaging device and method

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Embodiment Construction

[0017] The specific embodiments of the present invention will be described in further detail below in conjunction with the accompanying drawings.

[0018] The schematic structural diagram of the super-resolution acousto-optic modulation confocal imaging device of this embodiment is as follows: figure 1 As shown, the device includes a laser light source 1, a collimating beam expander 2, a focusing objective lens 3, a sample 4, a Raman-Ness diffraction modulated acoustic grating 5, and a first collecting objective lens 6 are sequentially placed along the light propagation direction of the laser light source 1. 2. The second collecting objective lens 7, the Raman-Ness diffraction demodulation acoustic grating 8, the pinhole 9, the light intensity detector 10, and the signal processing device 11; the grating of the modulated acoustic grating 5 and the demodulation acoustic grating 8 The type is the same, the space period is the same, it is set in the conjugate position of the object s...

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Abstract

A super-resolution acousto-optic modulation confocal imaging device and method belongs to the technical field of precise optical measurement. The device is provided with a Raman-Nath diffraction modulation acousto-optic grating and a Raman-Nath diffraction demodulation acousto-optic grating, which are the same in type and spatial period, and are driven ultrasonically at the same frequency in opposite directions in the conjugate position of the object space and the image space, and is provided with a signal processing device with a filtering or time integration function; the method is characterized in that light, passing through a sample, in a confocal system is subjected to space-time modulation by using the modulation acousto-optic grating, the modulation light is demodulated before entering into the demodulation acousto-optic grating so as to obtain a scanning spot instantaneous light intensity signal, and signal processing is carried out finally to obtain scanning spot light intensity. Even if no moving parts are required to be led into the device and the method, the spatial-cut-off frequency of the confocal system can be improved, even the implicit vector wave component can be enabled to participate in imaging, the space frequency domain bandwidth is widened, the transverse resolution of the system is improved remarkably, and the device and the method are especially suitable for increasing the numerical aperture and realizing the measuring field of the near-field implicit vector wave component during the far-field imaging.

Description

Technical field [0001] A super-resolution acousto-optic modulation confocal imaging device and method belong to the technical field of optical precision measurement. Background technique [0002] Optical microscopy is a non-destructive technology that is widely used in the fields of biology and material science. Confocal microscopy measurement technology has now become a three-dimensional optical microscopy technology suitable for micron and sub-micron scale measurement due to the tomographic capability of the reflective confocal microscopy system and the lateral resolution improved to 1.4 times that of ordinary microscopes with the same aperture. , Is an important branch in the field of microscopy. [0003] However, traditional confocal technology has always been limited by the principle that the numerical aperture of the traditional lens cannot exceed 1 due to the limitation of the lens aperture and the rapid attenuation of the near-field evanescent vector wave. Therefore, it ca...

Claims

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Application Information

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IPC IPC(8): G01B11/00G01B11/24
Inventor 邹丽敏王宝凯陈彦均谭久彬
Owner HARBIN INST OF TECH
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