A super-resolution moving grating confocal imaging device and method
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A technology for moving gratings and imaging devices, which is applied in the direction of using optical devices, measuring devices, instruments, etc., can solve the problems of super-resolution moving grating imaging methods that have not yet appeared.
Inactive Publication Date: 2015-12-02
HARBIN INST OF TECH
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[0005] So far, there have been no reports on the introduction of super-resolution moving grating imaging methods into confocal microscopy systems
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specific Embodiment 1
[0024] The modulating moving grating 5 and demodulating moving grating 8 used are both one-dimensional sinusoidal gratings with diffraction orders of 0 and ±1, and they move at a constant speed with equal and opposite speeds at the conjugate positions of the object space and the image space, focusing on The converging angles of the objective lens 3 and the first collecting objective lens 6 are equal, that is, the numerical apertures in the air are equal, and NA=0.1 is selected in this embodiment.
[0025] In this example, in order to keep the CTFs after band extension from overlapping, the spatial frequency of the grating used needs to satisfy
[0026] n 0 ≥4sin(α 0 ) / λ
[0027] where, v 0 is the spatial frequency of the grating, that is, the reciprocal of the spatial period of the grating; α 0 is the convergence angle of the focusing objective lens 3 and the first collecting objective lens 6; λ is the illumination light wavelength;
[0028] The normalized light intensity...
specific Embodiment 2
[0034] The difference between this embodiment and the specific embodiment 1 is that the modulating moving grating 5 and the demodulating moving grating 8 used are both second-level Dammann gratings with diffraction orders of 0, ±1 and ±2.
[0035] The normalized light intensity after filtered or integrated signal processing is:
[0036] I ( v , v x , v y ) = | [ 1 + 2 c o s ( 4 v x ) + 2 cos ( 2 v x ) ...
specific Embodiment 3
[0041] The difference between this embodiment and the specific embodiment 1 and the specific embodiment 2 is that the modulation moving grating 5 and the demodulating moving grating 8 are both two-dimensional sinusoidal gratings with x-axis and y-axis diffraction orders of 0 and ±1. The two-dimensional sinusoidal grating has the same spatial frequency as the x-axis direction and the y-axis direction, and moves at a constant speed with the same magnitude and opposite speed at the conjugate position of the object space and the image space, and the speed in the y-axis direction is the x-axis direction. 3 times above.
[0042] The normalized light intensity after filtered or integrated signal processing is:
[0043] I ( v , v x , v y ) = | [ A 0 ...
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Abstract
The invention belongs to the technical field of optical precision measurement, and provides a super resolution moving grating confocal imaging device and a confocal imaging method. The super resolution moving grating confocal imaging device is provided with a modulation moving grating, a demodulation moving grating and a signal processing device. The modulation moving grating and the demodulation moving grating are the same in type and in space cycle, and do linear motion or circumferential rotation at a conjugate position between an object space and an image space in constant speed in opposite directions. The signal processing device has a filtering or time integral function. The confocal imaging method includes the steps of using the modulation moving grating to carry out space-time modulation on light passing through samples in a confocal system, carrying out demodulation through the demodulation moving grating before the modulation light enters a light intensity detector, obtaining the instantaneous light intensity signals of scanning points, and carrying out signal processing to obtain the light intensity of the scanning points. The super resolution moving grating confocal imaging device and the confocal imaging method can improve spatial-cut-off frequency of the confocal system, even can enable implicit vector wave components to participate in imaging, broaden space frequency band width, and remarkably improve horizontal resolving ability, and is especially suitable for the measuring field for improving numerical aperture and achieving the measuring field of near-field implicit vector wave components imaging in a far field.
Description
technical field [0001] A super-resolution moving grating confocal imaging device and method belong to the technical field of optical precision measurement. Background technique [0002] Optical microscopy is a long-established and important non-destructive technique that is widely used in fields such as biology and materials science. Confocal microscopy is a three-dimensional optical microscopy technique suitable for micron and submicron scale measurements. The tomographic capabilities of reflection confocal microscopy systems make them very important in the field of 3D imaging. In the mid-to-late 1950s, the confocal microscope was invented by Minsky, and in 1977, C.J.R. Sheppard and A. Choudhury first clarified that the confocal microscope system, under the action of the point pinhole mask, makes the lateral resolution at the expense of the field of view Improve to 1.4 times that of ordinary microscopes with the same aperture. Since then, confocal microscopy has received...
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