Method for preventing and controlling melanophila picta pallas larvae by Sclerodermus pupariae carrying fusarium solani
A technology of fusarium solani and swollen-legged wasp, which is applied in the field of swollen-legged wasps carrying fusarium solani to control poplar larvae, can solve the problems of difficult control, pesticide residues, and poor control effect, and achieve Effect of increasing larval mortality
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Embodiment 1
[0026] The method for the prevention and treatment of the larvae of Populus chinensis by carrying Fusarium solani solani in the present invention, the method is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang, and the geographic coordinates are east longitude: 84 ° 50 ′08″, 45°22′13″ north latitude, is a pure poplar forest with high density planting, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the plant spacing is 1m, the row spacing is 2m, and the average population density per plant 13-20 heads, 75% of the worm strains will be verified. The specific steps are as follows:
[0027] a. Cultivation of entomological fungi: Fusarium solani was inoculated on a PDA plate at a temperature of 28°C and cultivated for 5 days. The purely cultured bacterial strain was inoculated on the slope of a PDA test tube of 15mm×150mm and cultivated at a temperature...
Embodiment 2
[0031] The method for the prevention and treatment of Populus chinensis by carrying Fusarium solani solani in the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang, and the geographic coordinates are east longitude: 84 ° 50′08″ , 45°22′13″ north latitude, it is a pure poplar forest with high density planting, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc. The spacing between plants is 1m, row spacing is 2m, and the average population density per plant is 13-20 Head, 75% of the worm strain rate is verified, and the specific steps are as follows:
[0032] a, entomological fungal culture: Fusarium solani was inoculated on a PDA plate at a temperature of 25° C. and cultivated for 6 days. The purely cultured bacterial strain was inoculated on the slope of a PDA test tube of 15 mm×150 mm at a temperature of 25° C. and cultivated for 1...
Embodiment 3
[0036] The method for the prevention and treatment of P. solani Fusarium solani by Fusarium solani of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. °22′13″, it is a pure poplar forest with high density planting, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the spacing between plants is 1m, the spacing between rows is 2m, and the average population density per plant is 13-20 heads. The rate of insect strains is 75% for verification, the specific steps are as follows:
[0037]a. Cultivation of entomological fungi: Fusarium solani was inoculated on a PDA plate at a temperature of 23°C and cultivated for 7 days. The purely cultured bacterial strain was inoculated on the slope of a PDA test tube of 15mm×150mm and cultivated at a temperature of 23°C for 13 days to produce a large number of conidia;
[0038] b. Bacteroide...
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