Method for preventing and controlling melanophila picta pallas larvae by Sclerodermus pupariae carrying fusarium solani

A technology of fusarium solani and swollen-legged wasp, which is applied in the field of swollen-legged wasps carrying fusarium solani to control poplar larvae, can solve the problems of difficult control, pesticide residues, and poor control effect, and achieve Effect of increasing larval mortality

Inactive Publication Date: 2014-09-10
FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method is reasonable and operable, and solves the problems of difficult control, poor contro...

Method used

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  • Method for preventing and controlling melanophila picta pallas larvae by Sclerodermus pupariae carrying fusarium solani
  • Method for preventing and controlling melanophila picta pallas larvae by Sclerodermus pupariae carrying fusarium solani
  • Method for preventing and controlling melanophila picta pallas larvae by Sclerodermus pupariae carrying fusarium solani

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Embodiment 1

[0026] The method for the prevention and treatment of the larvae of Populus chinensis by carrying Fusarium solani solani in the present invention, the method is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang, and the geographic coordinates are east longitude: 84 ° 50 ′08″, 45°22′13″ north latitude, is a pure poplar forest with high density planting, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the plant spacing is 1m, the row spacing is 2m, and the average population density per plant 13-20 heads, 75% of the worm strains will be verified. The specific steps are as follows:

[0027] a. Cultivation of entomological fungi: Fusarium solani was inoculated on a PDA plate at a temperature of 28°C and cultivated for 5 days. The purely cultured bacterial strain was inoculated on the slope of a PDA test tube of 15mm×150mm and cultivated at a temperature...

Embodiment 2

[0031] The method for the prevention and treatment of Populus chinensis by carrying Fusarium solani solani in the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang, and the geographic coordinates are east longitude: 84 ° 50′08″ , 45°22′13″ north latitude, it is a pure poplar forest with high density planting, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc. The spacing between plants is 1m, row spacing is 2m, and the average population density per plant is 13-20 Head, 75% of the worm strain rate is verified, and the specific steps are as follows:

[0032] a, entomological fungal culture: Fusarium solani was inoculated on a PDA plate at a temperature of 25° C. and cultivated for 6 days. The purely cultured bacterial strain was inoculated on the slope of a PDA test tube of 15 mm×150 mm at a temperature of 25° C. and cultivated for 1...

Embodiment 3

[0036] The method for the prevention and treatment of P. solani Fusarium solani by Fusarium solani of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. °22′13″, it is a pure poplar forest with high density planting, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the spacing between plants is 1m, the spacing between rows is 2m, and the average population density per plant is 13-20 heads. The rate of insect strains is 75% for verification, the specific steps are as follows:

[0037]a. Cultivation of entomological fungi: Fusarium solani was inoculated on a PDA plate at a temperature of 23°C and cultivated for 7 days. The purely cultured bacterial strain was inoculated on the slope of a PDA test tube of 15mm×150mm and cultivated at a temperature of 23°C for 13 days to produce a large number of conidia;

[0038] b. Bacteroide...

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Abstract

The invention relates to a method for preventing and controlling melanophila picta pallas larvae by scleroderma guani carrying fusarium solani. According to the method, the Sclerodermus pupariae is used for carrying fusarium solani to actively infect the melanophila picta pallas larvae, and the probability that the melanophila picta pallas larvae is infected with entomogenous fungi is increased while the fusarium solani parasitically lives on the Sclerodermus pupariae. Due to the synergistic composition effect of the Sclerodermus pupariae and the entomogenous fungi, the prevention and control effect on the melanophila picta pallas larvae can be enhanced, the death rate of the melanophila picta pallas larvae is increased, the melanophila picta pallas larvae have stronger selectivity and specialization for hosts and have no toxic effect on higher plants and animals, and the environment is not polluted. The method has a sustainable control function, is reasonable, and strong in operability. According to the method, the problems of high difficulty of preventing and controlling melanophila picta pallas, poor preventing and controlling effect, pesticide residues and the like are solved, and a new way is provided for the prevention and control for the melanophila picta pallas larvae.

Description

technical field [0001] The present invention relates to a kind of Yang Shi Ban Ji Ding Melanophila picta Pallas larval control method, specifically related to Scleroderma aureus Sclerodermus pupariae Fusarium solani Fusarium solani The method of preventing and controlling the larvae of Poplar ten spot. Background technique [0002] Yang Shiban Ji Ding Melanophila picta Pallas primary larvae do damage between the bark and xylem, feed on the phloem tissue of the tree, gradually penetrate into the cambium as the age of the larva grows, and the insect feces are not discharged outside, causing the death of poplars, willows and other damaged trees, which are hidden It is difficult to achieve a good control effect with conventional control methods. When the damage is serious, the population density of each plant can reach 50-60, or even as high as 100. The rate of damaged plants can reach 60-90%. The death of forests destroys the forests invested and painstakingly managed ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P7/04C12R1/77
Inventor 岳朝阳张新平阿里木·买买提克热曼·赛米陈晶晶张静文刘爱华焦淑萍
Owner FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
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