UCH320 protein and application of coding gene thereof in adjusting and controlling plant growth and development

A technology encoding gene and protein is applied in the application field of UCH320 protein and its encoding gene in regulating plant growth and development, which can solve the problems of long cycle, slow effect, unable to meet the urgent needs of production and development, etc., and improve the seed setting rate. , the effect of seed weight reduction

Active Publication Date: 2014-11-05
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For a long time, people have been using conventional breeding methods to select hybrid crops. These methods have a long cycle and slow results, and cannot meet the urgent needs of production development.

Method used

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  • UCH320 protein and application of coding gene thereof in adjusting and controlling plant growth and development
  • UCH320 protein and application of coding gene thereof in adjusting and controlling plant growth and development
  • UCH320 protein and application of coding gene thereof in adjusting and controlling plant growth and development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Embodiment 1, the acquisition and identification of UCH320 transgenic plants

[0092] The UCH320 gene involved in this example is derived from rice (Oryza.sativa L.), and its cDNA sequence is shown in sequence 2 in the sequence table. Sequence (ORF); Sequence 2 encodes the protein (UCH320 protein) shown in Sequence 1 in the Sequence Listing, and Sequence 1 consists of 229 amino acid residues.

[0093] 1. Construction of RNAi expression vector pCAM23A-UCH320

[0094] Design the following RNAi primer sequences according to sequence 2 in the sequence listing:

[0095] RNAi-23A320-F: 5'-cc ACT AGT ATG GAG GAT GCT CAT TCC-3' (underlined is the recognition sequence of the enzyme cutting site Spe I, followed by the 534-551th position of sequence 2)

[0096] RNAi-23A320-R: 5'-Tc GGA TCC CAC AAC TTT CGA AAG AGC-3' (underlined is the recognition sequence of the restriction site BamH I, followed by the reverse complement of the 771-788th position of sequence 2)

[0097] U...

Embodiment 2

[0135] Embodiment 2, functional identification of transgenic rice

[0136] Positive T was identified in Example 1 with 24 strains 1 The transgenic rice transgenic plants transformed into the RNAi expression vector pCAM23A-UCH320, the non-transgenic wild type rice variety Zhonghua No. 11, and the control plants obtained in Example 1 transformed into the pCAM23A empty vector were used as experimental materials. The seeds of each experimental material were sown in petri dishes for accelerated germination (80-100 seeds were sown for each experimental material), and the accelerated seedlings were transplanted into flower pots to emerge, and then transferred to large fields in the suburbs of Beijing for growth. After harvesting the ears of each experimental material plant, it was analyzed and identified in the following aspects:

[0137] 1. Analysis of rice ear phenotype

[0138] The results showed that compared with the non-transgenic wild-type rice variety Zhonghua 11, 24 positi...

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Abstract

The invention discloses UCH320 protein and an application of encoding code thereof in adjusting and controlling plant growth and development. The application provided by the invention is an application of protein consisting of amino acid sequences shown by a sequence 1 in a sequence table or coding genes thereof in adjusting and controlling the plant growth and development. The plant growth and development is specifically embodied in at least one of (1) and (2): (1) the fructification rate of the seed; (2) the weight of the seed. Proven by experiment, in the development process of rice, the expression level of UCH320 protein in the rice is reduced by an RNA (Ribonucleic Acid) interference technology, the rice seed can be caused to show the following phenotype: compared with the wild type rice seed, the fructification rate is reduced, the fertile seed is also long and thin, and the weight of the seed is also obviously reduced. The invention lays foundation for finding out simpler ideas and methods for creating crops with high-yield characters.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and relates to the application of a UCH320 protein and its coding gene in regulating plant growth and development. Background technique [0002] Since the 1970s, the utilization of heterosis has made a significant contribution to rice production in my country. Facing the new and important demand of "high yield, high quality, high efficiency, safety, and ecology" put forward by my country's economic development for agricultural production, whether to further explore the application potential of heterosis has become a severe challenge for contemporary scientists. [0003] The formation of heterosis is based on the combination of two different parents. To further explore its potential on the basis of existing applications, in addition to strengthening the research on the formation mechanism of heterosis, it is also urgent to establish effective methods to create male sterility trait...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84C12N1/21C12N5/10C12N15/113A01H5/00
CPCC07K14/415A01N37/46A01N65/44C12N15/8261Y02A40/146
Inventor 王东辉白书农刘娜许智宏
Owner PEKING UNIV
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