The invention discloses a castration method for rice hyberdization, belongs to the technical field of crop breeding and particularly relates to a new castration method for rice hyberdization based on spikelet clipping and water spraying. The castration method for rice hyberdization comprises the following steps: 1, determining reasonable sowing time; 2, selecting rice plants; 3, pruning spikes of whole plants; 4, performing castration based on spikelet clipping and water spraying; and 5, performing pollination timely and efficiently. According to the castration method disclosed by the invention, the problems of high labor intensity, incomplete castration and hyberdization failure due to pistil damage in the past rice hyberdization work are solved, and the technology used in the castration method has the advantages of labor and cost economy, simplicity and convenience in operation, high hyberdization success rate, economy, environment friendliness and the like, and is highly popular among scientific research workers on breeding, and has a wide application prospect.