Plant temperature-sensitive sterile mutant tms15 and application thereof

Abstract

The invention discloses a plant temperature-sensitive sterile gene mutant tms15 and application thereof. A nucleotide sequence of the plant temperature-sensitive sterile gene mutant tms15 comprises a nucleotide sequence as shown in SEQ ID No.1 in a sequence table. The inventor carries out EMS mutagenesis on the middle flower 11 to screen a thermo-sensitive genic male sterile line tms15, and the thermo-sensitive genic male sterile line tms15 is subjected to EMS mutagenesis under a high temperature condition (gt; the male sterility phenotype is shown under the low-temperature condition (22-24 DEG C), and the fertility is recovered under the low-temperature condition (22-24 DEG C). Cytology results show that spore-forming cells in tms15 at high temperature are excessively proliferated, tapetum cells are also abnormally vacuolated, and normal pollen cannot be formed. After low-temperature treatment, normal proliferation of spore-forming cells and normal development of tapetum cells are recovered to a certain extent. The mutation of one basic group in the LRR section in the coding region of the gene enables the amino acid coded at the site to be changed from valine to glutamic acid, and the mutation enables the expression of the TMS15 gene to be obviously up-regulated compared with that of a wild type. The unique characteristic of the gene can provide new theoretical basis and application value for cross breeding of two lines.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to a plant temperature-sensitive sterile mutant and its application, which can restore fertility under specific conditions. Background technique [0002] In agricultural production, plant male sterility provides an important approach and germplasm resources for the utilization of heterosis. [0003] Heterosis refers to the F 1 The generation plants are stronger than the parents in terms of total biomass and seed setting. Mr. Yuan Longping proposed the concept of using rice heterosis, which opened the theoretical process of hybrid rice breeding in my country. In the 1970s, Yuan Longping's team discovered a wild-aborted strain whose pollen was completely aborted. Through the efforts of rice breeders across the country, wild defeat was successfully applied to the breeding of three-line hybrid rice, which made hybrid rice popularized and applied nationwide (Wu Bi et al., 2018). The succes...

AI Technical Summary

Problems solved by technology

[0009] At present, there are more than 20 GMS lines based on the response to light and temperature, but the cloned genes are still very limited. Although a certain degree of breakthrough has been achieved, further in-depth research is needed. Resources are relatively scarce, so it is necessary to discover new photothermosensitive gene resources

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