Tissue culture technique of plumbago auriculata

A technology of tissue culture and snowflakes, which is applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient, lack of materials, long cycle, etc., and achieve the effect of increasing multiplication rate, low multiplication coefficient and low seed setting rate

Inactive Publication Date: 2015-06-10
刘祖英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of materials, people's understanding and application of blue snowflakes are still very limited.
At present, Lanxuehua mainly propagates seedlings by sowing, cuttings and other propagation methods, which have disadvantages such as low reproduction coefficient, long cycle, and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Disinfection of explants: cut young shoots that are growing well and free from diseases and insect pests, soak them in washing powder solution for 50 minutes, rinse them with running water for 1 hour, disinfect them with 75% ethanol for 30 seconds in an ultra-clean workbench, and wash them 3 times with sterile water. Then sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water for 4 times, and absorb the water with sterile filter paper for later use.

[0018] (2) Single bud induction: Cut the explants treated in step (1) into stem segments about 1.0 cm long, with the stem nodes as the boundary, retaining 0.3 cm in the upper half and 0.7 cm in the lower half, each Each stem segment retains one stem node. Inoculate the stem segments into the induction medium for single bud induction. After inoculation, first culture in total darkness at 25°C for 5 days, and then place it under the condition of 12 hours of light per day, and culti...

Embodiment 2

[0023] (1) Disinfection of explants: Cut young shoots with good growth and no pests and diseases, soak them in washing powder solution for 80 minutes, rinse them with running water for 3 hours, disinfect them with 75% ethanol for 50 seconds in an ultra-clean workbench, and wash them 3 times with sterile water. Then sterilize with 0.1% mercuric chloride solution for 12 minutes, rinse with sterile water for 5 times, and absorb the water with sterile filter paper for later use.

[0024] (2) Single bud induction: Cut the explants treated in step (1) into stem segments about 1.0 cm long, with the stem nodes as the boundary, retaining 0.3 cm in the upper half and 0.7 cm in the lower half, each Each stem segment retains one stem node. Inoculate the stem segments into the induction medium for single bud induction. After inoculation, first culture in total darkness at 26°C for 6 days, and then place it under the condition of 13 hours of light per day and 2000lx light intensity f...

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Abstract

The invention discloses a tissue culture technique of plumbago auriculata. As plumbago auriculata is low in setting percentage, the seeds of plumbago auriculata are relatively expensive, plumbago auriculata is further low in expanding propagation to result in that the existing traditional propagation method cannot meet the application requirements of plumbago auriculata any more, however, the tissue culture technique of plumbago auriculata is capable of overcoming the shortcomings of the traditional propagation method and capable of producing good-quality plumbago auriculata variety on a large scale in relatively short time. According to the tissue culture technique, a plumbago auriculata in-vitro regeneration system is established by taking the tender twigs of plumbago auriculata as the explants and by virtue of the processes of explant processing, single bud induction, multiple shoot proliferation, rooting, acclimatization and transplant and the like; as a result, the proliferation ratio of plumbago auriculata can be greatly increased, and technical support is provided for the industrialized production of the tissue culture of plumbago auriculata.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a blue snowflake tissue culture technology. Background technique [0002] Blue Snowflake ( Plumbago auriculata ), also known as Blue Flower Dan, Blue Flower Rocky Pine, Blue Jasmine, and Blue Snow Dan, is a perennial evergreen shrub of the family Plumbaceae. Originally produced in South Africa, it is currently cultivated in the tropics and subtropics of the world. It is a new type of flower with excellent ornamental and medicinal value. Blue snowflakes are managed extensively, have few pests and diseases, are resistant to high temperature and high humidity, grow vigorously, and have a long viewing period. Its leaves are emerald green and its flowers are light and elegant, giving people a cool feeling in hot summer. It can be potted to decorate the living room and balcony. Adult plants have a large crown and are resistant to pruning. They are s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘祖英
Owner 刘祖英
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