Method for improving degradation capability of Pseudomonas sp on petroleum hydrocarbons

A technology of pseudomonas and petroleum hydrocarbons, applied in biochemical equipment and methods, electric/wave energy treatment of enzymes, and electric/wave energy treatment of microorganisms, etc., can solve the problem of narrow variation spectrum, low degradation efficiency, high degradation cost, etc. problem, to achieve the effects of high mutation frequency, good mutation stability, and wide variation spectrum

Inactive Publication Date: 2013-10-02
XIAN TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using Pseudomonas to degrade petroleum hydrocarbons is an effective method, but the main problems at present are high degradation cost due to its low degradation efficiency, narrow mutation spectrum, low mutation rate, and complicated operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Dilute Pseudomonas in sterile water, take out 0.1ml of the solution and spread it evenly in a petri dish;

[0030] 2) Sterilize the auxiliary vacuum chamber of the ionizing radiation system with alcohol, and put the prepared sample into the auxiliary vacuum chamber of the ionizing radiation vacuum system;

[0031] 3) Open the gate valve between the auxiliary vacuum chamber and the main vacuum chamber, and quickly draw the auxiliary vacuum chamber to 8*10 -4 Pa;

[0032] 4) Turn on the nitrogen ion radiation source, adjust the ion energy and radiation dose to 20Kev and 0.75*1016 ions / mm respectively 2 ;

[0033] 5) Carry out nitrogen ion radiation, turn off the nitrogen ion radiation source after completion, close the gate valve, inflate the auxiliary vacuum chamber, and take out the Pseudomonas culture dish;

[0034] 6) Wash Pseudomonas from the culture dish with sterile water, dilute to a certain concentration, and then screen the colonies;

[0035] 7) Test the ...

Embodiment 2

[0037] 1) Dilute Pseudomonas in sterile water, take out 0.3ml solution and evenly spread it on the petri dish;

[0038] 2) Sterilize the auxiliary vacuum chamber of the ionizing radiation system with alcohol, and put the prepared sample into the auxiliary vacuum chamber of the ionizing radiation vacuum system;

[0039] 3) Open the gate valve between the auxiliary vacuum chamber and the main vacuum chamber, and quickly draw the auxiliary vacuum chamber to 2*10 -3 Pa;

[0040] 4) Turn on the nitrogen ion radiation source, adjust the ion energy and radiation dose to 15Kev and 1.25*1016 ions / mm respectively 2 ;

[0041] 5) Carry out nitrogen ion radiation, turn off the nitrogen ion radiation source after completion, close the gate valve, inflate the auxiliary vacuum chamber, and take out the Pseudomonas culture dish;

[0042] 6) Wash Pseudomonas from the culture dish with sterile water, dilute to a certain concentration, and then screen the colonies;

[0043] 7) Test the varia...

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PUM

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Abstract

The invention relates to a method for improving the degradation capability of Pseudomonas sp on petroleum hydrocarbons. Petroleum hydrocarbons can be effectively degraded by adopting Pseudomonas sp, and the main problems of high degradation cost, narrow variation frequency spectrum, low variation rate and complex operation exist at present. The method for improving the degradation capability of Pseudomonas sp on petroleum hydrocarbons is characterized in that ion beams generated by an ion radiation system are injected to the Pseudomonas sp to improve the degradation capability of the Pseudomonas sp on the petroleum hydrocarbons. The method adopting the ion radiation induced mutation breeding has the advantages of convenient adjustment and control of the ion energy and the injection dosage, strong adaptability, high variation frequency, wide variation spectrum and good variation stability.

Description

technical field [0001] The invention relates to the field of microbial breeding, in particular to a method for improving the petroleum hydrocarbon degradation ability of pseudomonas. Background technique [0002] Petroleum is a mixture of natural organic compounds with very complex components. The main component is liquid hydrocarbons. Petroleum hydrocarbons are a kind of chemical polymer organic matter. Many hydrocarbons are toxic and seriously pollute the environment. It is imperative to take effective measures to control them. must do. Using Pseudomonas to degrade petroleum hydrocarbons is an effective method, but the main problems at present are high degradation cost due to its low degradation efficiency, narrow mutation spectrum, low mutation rate, and complicated operation. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for improving the petroleum hydrocarbon degradation ability of Pseudomonas by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N15/01
Inventor 蔡长龙廉振民梁海锋马睿彭渝丽刘正利严一心
Owner XIAN TECH UNIV
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