Ageratina adenophora water culture method

A technique for cultivating E. japonica and a cultivation method, which is applied in the field of E. japonica hydroponics, and can solve the problems of poor ventilation, difficulty in recovering root systems, external environmental hazards and the like.

Inactive Publication Date: 2013-10-16
HUNAN UNIV OF SCI & ENG
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Problems solved by technology

[0004] 1. It is difficult to recover the complete root system from the samples of the soil culture medium, which is not conducive to the requirements of some studies on the root system of Eupatorium adenophorum. At the same time, because the root system is stuck in the soil, the aeration is poor, and the soil is easy to compact, resulting in too long experiment time or experimental results. not obvious
[0005] 2. In the soil culture medium, the root system of Eupatorium adenophorum is easy to rot, and it is difficult to obtain uniform experimental materials, which causes large errors in the experiment
[0006] 3. As a vicious invasive weed, the cultivation site of Eupatorium adenophorum must be strictly controlled. Soil cultivation may cause seeds and rooted branches to spread outside, causing potential harm to the external environment
[0008] Through document retrieval, do not see the public report identical with the inventive method

Method used

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Embodiment Construction

[0021] Use plastic culture bottles with length, width and height of 30cm, 23cm and 4.5cm as the culture container, inject 1L1 / 2 times of hydroponic nutrient solution into each pot, and fix the seedlings with a 1.5cm thick foam board and sponge with holes matrix.

[0022] Before transplanting the seedlings, select Eupatorium adenophorum seeds of the same size and sterilize them with 0.1% mercuric chloride solution for 20 minutes, wash them with sterile water for 5 times, each time for 30 seconds, and then soak the seeds in sterile water at 25°C for 12 hours; The soaked seeds were placed in a sterilized Petri dish lined with two layers of filter paper, and 5 mL of sterile water was added, and placed in a light incubator for cultivation (constant temperature 25°C, day:night 14h:10h, relative humidity 70% ), add water at intervals, and wait until the height of the seedling grows to two cotyledons; finally, wrap the base of the uniformly growing seedlings with a sponge, gently pick...

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Abstract

The invention relates to an ageratina adenophora water culture method which comprises the following steps: 1, sprouting seeds, namely culturing disinfected ageratina adenophora seeds into seedlings in an incubator; 2, making a culturing device; 3, transplanting the seedlings, namely placing the seedings obtained in the step 1 into the culturing device obtained in the step 2; 4, culturing the seedlings, namely placing one second time of water culture nutrient solution into the culturing device, replacing with 1 time of water culture nutrient solution to continuously culture after culturing for 9 days, and replacing the nutrient solution at intervals of 3 days. The method is convenient to obtain the consistent and high-quality water-cultured seedlings and can provide reliable material resources for search on the aspects of invasive mechanism, chemical and biological prevention and treatment, molecular biology, physiological ecology and the like of ageratina adenophora.

Description

1. Technical field: [0001] The method of the invention relates to a hydroponic method of Eupatorium adenophorum. 2. Background technology: [0002] Eupatorium adenophorum (Eupatorium adenophorum Spreng) is a worldwide malignant weed, which is a clustered semi-shrub perennial herb of the genus Eupatorium adenophorum in the family Asteraceae. At present, it has been widely distributed in Yunnan, Guizhou, Sichuan, Guangxi, Tibet and other places. It has spread and caused disasters in the invaded areas, causing serious harm to the local ecological environment, economic development and human health. It has become a malignant weed and has grown to be difficult to control. Degree. According to reports, there is no effective method to completely control Eupatorium adenophorum, and the mechanism of rapid spread of Eupatorium adenophorum is not very clear. Therefore, it is particularly important to strengthen the research on the invasion mechanism, chemical and biological control, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
Inventor 刘小文袁先友张敏齐成媚蒋海明骆鹰戴永强
Owner HUNAN UNIV OF SCI & ENG
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