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Use of ides as biomarkers for scalp disorders

A use, technology of the scalp, applied in its field as an active agent

Active Publication Date: 2017-05-24
LOREAL SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, many unknown factors are still uncertain as to the underlying mechanisms and factors involved in the desquamation of the skin and especially the factors involved in the onset of dandruff

Method used

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  • Use of ides as biomarkers for scalp disorders
  • Use of ides as biomarkers for scalp disorders
  • Use of ides as biomarkers for scalp disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0298] Expression of IDE in the scalp

[0299] 1- Materials and methods

[0300] A 2D differential electrophoretic proteome analysis was performed using non-invasive samples of dandruff-free scalp and scalp with dandruff.

[0301] The study was carried out on 6 volunteers without dandruff (adhesive dandruff scale from 0 to 0.25) and 6 volunteers with dandruff (adherent dandruff scale from 3.25 to 4). The volunteers were short-haired men aged 36 to 39. The grading of the dandruff condition of the volunteers was carried out by an expert according to a standard classification providing a scoring scale between 0 and 5 for the attached dandruff.

[0302]Sampling was performed through a corneodisc (code GODS100, CuDerm) over an area of ​​2 cm. Four saturated horny discs were generated for each sample. Soluble protein was extracted and put into native buffer (TBS, 1M NaCl, 1% Triton X100).

[0303] After filtration, the protein was precipitated by adding acetone. Protein pell...

Embodiment 2

[0314] Confirmation by Western blot of expression of IDE in skin

[0315] 1-Materials and methods

[0316] The concentrations of the samples described above in Example 1 were aligned and 2 μg were placed in the wells of a 10-20% standard gel (BioRad). Therefore, proteins were separated by SDS-PAGE electrophoresis. After semi-dry transfer on PVDF membranes according to standard protocols, proteins were incubated overnight at 4°C with primary antibodies against the protein of interest. A second incubation was then performed with a secondary antibody (conjugated to peroxidase) against the primary antibody.

[0317] Electrochemiluminescence kits are used to visualize target proteins.

[0318] Images were acquired using FluorSmax (Biorad) and the band was quantified using Quantity-One software (Biorad). A commercially available anti-IDE antibody was used at a dilution of 1 / 5000 (reference AB28560, AbCam).

[0319] 2- Results

[0320] image 3 The obtained western blot im...

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Abstract

The subject matter of the present invention is in particular the amino acid sequence of the IDE or an analog or fragment thereof or at least one nucleic acid sequence encoding the sequence as a biomarker or active agent for dandruff disorders on the scalp.

Description

technical field [0001] The present invention relates to the field of cosmetic and / or therapeutic biomarkers and targets for dandruff conditions of the skin, especially for the scalp, and also to their use as active agents. Background technique [0002] For the purposes of the present invention, the term "skin" refers to all epidermis of the human body, including mucous membranes and areas of skin covered with body hair or hair. In particular, the skin considered in the present invention is preferably the skin of the lips and face, the skin of the neck or the scalp, even more preferably the skin of the neck. [0003] Skin is a tissue in which cells are connected together and integrally attached to each other. It forms the outer layer that includes the sebaceous or sweat glands and hair follicles. The skin, especially the scalp, is an epidermis that undergoes continuous regeneration. Regeneration or desquamation is a coordinated and finely regulated process that results in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C07K7/06C07K7/08
CPCC07K7/06C07K7/08G01N33/6893G01N2333/96486G01N2800/20C12N9/6489A61P17/00C12Q1/34
Inventor C·德拉特P·希尔文D·伯纳德
Owner LOREAL SA
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