SNP molecular marker related to pig carcass trait and application thereof

A technology of molecular markers and carcasses, which is applied in the direction of recombinant DNA technology, measurement/testing of microorganisms, DNA/RNA fragments, etc., and can solve the problem of few molecular markers
CN103421769BInactive Publication Date: 2015-02-04HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2015-02-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to technical field of preparations of livestock molecular markers, and particularly to preparation and application of an SNP molecular marker related to a pig carcass trait. The preparation comprises the following steps: cloning in pig Col2A1 gene to obtain a gene segment used as the molecular marker and related to the pig carcass trait, wherein the nucleotide sequence is as shown in a sequence table of SEQ ID NO:1, and SmaI-RFLP polymorphism is caused by the base substitution of A276-G276 in a 276bp point of the SEQ ID NO:1. The invention also discloses a method for the preparation of the molecular marker related to the pig carcass trait and the application of the prepared molecular marker in the investigation of polymorphism of the pig carcass trait to provide a new mark for marker assisted selection of a pig.
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Description

technical field

[0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to a method for predicting pig carcass traits by using COL2A1 gene single nucleotide polymorphism and molecular markers thereof. The method is related to SNP molecular markers related to pig carcass traits. Background technique

[0002] Molecular marker-assisted selection is an important application of genetic engineering in modern livestock breeding. The method of molecular markers can not only shorten the breeding time limit, but also greatly reduce the human and material resource consumption of breeding; The application potential in animal breeding has been greatly improved (Lu Shaoxiong, Wu Changxin., 2000). Molecular markers for assisted selection include protein markers, microsatellite markers, SNP markers, etc. Single nucleotide polymorphism (single nucleotide polymorphism, SNP) mainly refers to the DNA sequence polymorphism caused ...

Claims

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