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Culture medium composition for strawberry tissue culture

A tissue culture and culture medium technology, applied in plant regeneration, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the development of strawberries, undernutrition, and overnutrition, and improve the rooting rate and shoot tip survival rate of strawberries. The effect of overcoming nutrient excess or nutrient deficiency and optimizing nutrient supply

Active Publication Date: 2013-12-11
赫聪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Strawberry is the earliest mature fruit in a single point. It can be harvested and put on the market in late spring and early summer. It has a long industrial chain and can be processed into various foods such as fruit juice, jam, beverages, cakes, and preserved fruits. However, strawberry production has long been They all rely on the traditional soil cultivation ramet propagation method to produce seedlings. The problems of traditional soil cultivation methods such as high labor intensity, short fruiting period, and continuous cropping obstacles have become increasingly prominent, which limits the further development of strawberry. With the rapid development of facility horticulture, no Soil cultivation technology is being popularized and applied in a large area and on a large scale. Some safe, high-quality, convenient and good composite cultivation substrates are constantly being developed, synthesized and used. The substrate required for growth is prepared separately from the substrate required for induced growth and multiplication growth, resulting in excess nutrients during the basic growth process and possible nutritional deficiencies in the later induced growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A medium composition for strawberry tissue culture: comprising basal medium, induction medium and propagation medium: each component and the contained weight are as follows:

[0022] (1) Basic medium: 1 / 4 of the mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 3g / L, pH adjusted to 5.0;

[0023] (2) Induction bud differentiation medium: a mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 6g / L, 2,4-D0.3mg / L, 6BA0.3mg / L, pH adjusted to 5.5;

[0024] (3) Rooting induction medium: 1 / 2 mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 4g / L, NAA0.2mg / L, 6BA0.2mg / L, pH adjusted to 5.5;

[0025] (4) Propagation medium: the mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 6g / L, 2,4-D0.2mg / L, NAA0.1mg / L, 6BA0.2mg / L L, pH adjusted to 5.5.

Embodiment 2

[0027] A medium composition for strawberry tissue culture: comprising basal medium, induction medium and propagation medium: each component and the contained weight are as follows:

[0028] (1) Basic medium: 1 / 4 of the mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 5g / L, pH adjusted to 6.0;

[0029] (2) Induction bud differentiation medium: a mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 8g / L, 2,4-D 0.3mg / L, 6BA 0.3mg / L, pH adjusted to 6.0;

[0030] (3) Rooting induction medium: 1 / 2 mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 6g / L, NAA0.2mg / L, 6BA0.2mg / L, pH adjusted to 6.0 ;

[0031] (4) Propagation medium: the mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 8g / L, 6g / L, 2,4-D0.2mg / L, NAA0.1mg / L, 6BA 0.2mg / L, pH adjusted to 6.0.

Embodiment 3

[0033] A medium composition for strawberry tissue culture: comprising basal medium, induction medium and propagation medium: each component and the contained weight are as follows:

[0034] (1) Basic medium: 1 / 4 of the mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 4g / L, pH adjusted to 5.5;

[0035] (2) Induction bud differentiation medium: a mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 7g / L, 2,4-D 0.3mg / L, 6BA 0.3mg / L, pH adjusted to 5.7;

[0036] (3) Rooting induction medium: 1 / 2 mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 5g / L, NAA0.2mg / L, 6BA0.2mg / L, pH adjusted to 5.7 ;

[0037] (4) Propagation medium: the mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 7g / L, 6g / L, 2,4-D0.2mg / L, NAA0.1mg / L, 6BA 0.2mg / L, pH adjusted to 5.7.

[0038] In the above examples 1-3, the solvent of the improved MS culture medium is water, and the components include macroelements, trace elements, iron salts and organic matt...

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PUM

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Abstract

The invention discloses culture medium composition for strawberry tissue culture. During the whole strawberry growth process, matrix for basic growth and matrix for inducing growth and propagation growth are separately prepared, and with the matching of components in different proportions for the culture media, nutrient supply of the culture medium is optimized, the nutrient balance during the strawberry growth process is ensured, defects of overnutrition or undernutrition, which are easy to appear in each phase of the traditional culture medium, are effectively overcome, the rooting percentage of the strawberry and the stem survival rate are increased, and the seedling quality is improved.

Description

technical field [0001] The invention relates to the formulation of crop culture medium, in particular to a medium composition for strawberry tissue culture. Background technique [0002] Strawberry is a perennial evergreen herb of the Rosaceae family Strawberry. It is a worldwide fruit. It also has certain medical value and has a certain curative effect on gastrointestinal diseases and anemia. Strawberry is the earliest ripe fruit in Yidian. It can be harvested and put on the market in late spring and early summer. It has a long industrial chain and can be processed into various foods such as fruit juice, jam, beverage, cake and preserved fruit. However, strawberry production has a long history. All rely on the traditional soil cultivation method to produce seedlings. The traditional soil cultivation method is labor-intensive, the fruiting period is short, and the problems of continuous cropping are becoming more and more prominent, which restricts the further development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 霍恒志糜林万春雁李金凤陈丙义陈雪平耿国民
Owner 赫聪
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