Culture medium composition for strawberry tissue culture
A tissue culture and culture medium technology, applied in plant regeneration, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the development of strawberries, undernutrition, and overnutrition, and improve the rooting rate and shoot tip survival rate of strawberries. The effect of overcoming nutrient excess or nutrient deficiency and optimizing nutrient supply
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Embodiment 1
[0021] A medium composition for strawberry tissue culture: comprising basal medium, induction medium and propagation medium: each component and the contained weight are as follows:
[0022] (1) Basic medium: 1 / 4 of the mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 3g / L, pH adjusted to 5.0;
[0023] (2) Induction bud differentiation medium: a mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 6g / L, 2,4-D0.3mg / L, 6BA0.3mg / L, pH adjusted to 5.5;
[0024] (3) Rooting induction medium: 1 / 2 mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 4g / L, NAA0.2mg / L, 6BA0.2mg / L, pH adjusted to 5.5;
[0025] (4) Propagation medium: the mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 6g / L, 2,4-D0.2mg / L, NAA0.1mg / L, 6BA0.2mg / L L, pH adjusted to 5.5.
Embodiment 2
[0027] A medium composition for strawberry tissue culture: comprising basal medium, induction medium and propagation medium: each component and the contained weight are as follows:
[0028] (1) Basic medium: 1 / 4 of the mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 5g / L, pH adjusted to 6.0;
[0029] (2) Induction bud differentiation medium: a mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 8g / L, 2,4-D 0.3mg / L, 6BA 0.3mg / L, pH adjusted to 6.0;
[0030] (3) Rooting induction medium: 1 / 2 mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 6g / L, NAA0.2mg / L, 6BA0.2mg / L, pH adjusted to 6.0 ;
[0031] (4) Propagation medium: the mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 8g / L, 6g / L, 2,4-D0.2mg / L, NAA0.1mg / L, 6BA 0.2mg / L, pH adjusted to 6.0.
Embodiment 3
[0033] A medium composition for strawberry tissue culture: comprising basal medium, induction medium and propagation medium: each component and the contained weight are as follows:
[0034] (1) Basic medium: 1 / 4 of the mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 4g / L, pH adjusted to 5.5;
[0035] (2) Induction bud differentiation medium: a mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 7g / L, 2,4-D 0.3mg / L, 6BA 0.3mg / L, pH adjusted to 5.7;
[0036] (3) Rooting induction medium: 1 / 2 mixture of modified MS, agar and gellan gum (mass ratio of 3:1) 5g / L, NAA0.2mg / L, 6BA0.2mg / L, pH adjusted to 5.7 ;
[0037] (4) Propagation medium: the mixture of modified MS, agar and gellan gum (mass ratio is 3:1) 7g / L, 6g / L, 2,4-D0.2mg / L, NAA0.1mg / L, 6BA 0.2mg / L, pH adjusted to 5.7.
[0038] In the above examples 1-3, the solvent of the improved MS culture medium is water, and the components include macroelements, trace elements, iron salts and organic matt...
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