A method for detecting hepatitis B surface antigen by inductively coupled plasma mass spectrometry

A technology of hepatitis B surface antigen and inductive coupling, applied in the field of mass spectrometry, can solve the problems of limited selection of markers, narrow detection range, radiation hazards, etc., and achieve the effect of excellent accuracy, rapid detection, and wide linear range
CN103439501BInactive Publication Date: 2016-03-23HIGH & NEW TECH RES CENT OF HENAN ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HIGH & NEW TECH RES CENT OF HENAN ACAD OF SCI
Publication Date
2016-03-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a method for detecting hepatitis B surface antigen (HBsAg) by inductively coupled plasma mass spectrometry (ICP-MS), which involves covalently linking hepatitis B virus surface antibody (HBsAb) to metal colloid particles with activated carboxyl groups , the HBsAg in the serum and the colloidal particle-labeled HBsAb combine to form an immune complex through a specific reaction; the colloidal particles are dissociated from the immune complex under acidic conditions, and then the metal ion signal is detected by ICP-MS, thereby indirectly detecting HBsAg . Since each colloidal particle can be connected with one or more antibody molecules, the sensitivity of the detection is greatly improved; this method overcomes the disadvantages of low accuracy of the traditional colloidal gold immunochromatography and enzyme-linked immunoassay. It has excellent accuracy and high sensitivity, and the immunoassay detection is convenient and rapid, which broadens the application of ICP-MS in immunoassay.
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Description

technical field

[0001] The invention belongs to the field of mass spectrometry analysis, in particular to a method for detecting hepatitis B surface antigen by inductively coupled plasma mass spectrometry. Background technique

[0002] China is a country with a high incidence of hepatitis B virus. According to epidemiological surveys, the incidence rate in the population is relatively high, and the surface antigen carrying rate is about 15%. The experimental diagnosis of hepatitis B virus has important reference significance for its treatment and prevention. Hepatitis B surface antigen (HBsAg) is the first serological marker to appear after HBV infection, and it is also one of the important indicators for diagnosis. For reasons such as testing costs, most domestic clinical laboratories use enzyme-linked immunosorbent assay (ELISA) to detect HBsAg. However, for samples with low HBsAg concentration, the detection sensitivity is often low due to methodological reasons, and t...

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