Method of measuring anthocyanins in ornamental Chinese flowering apple

Abstract

The invention discloses a method of measuring anthocyanins in ornamental Chinese flowering apple and belongs to the field of flower measurement. The method includes the steps of 1, placing leaves of ornamental Chinese flowering apple which is fast-frozen with liquid nitrogen into a mortar, adding silica sand and insoluble PVPP, and adding liquid hydrogen before grinding to obtain powder; 2, transferring the powder into a test tube, adding anthocyanins extract, performing ultrasonic extraction at 45 DEG C for 60 minutes to obtain extracted anthocyanins liquid; 3, purifying the extracted anthocyanins liquid, centrifuging at the speed of 12000 / m for 3 minutes to obtain supernate, and loading the supernate in an Agilent feeding bottle for HPLC detection; 4, feeding a sample, subjecting the Agilent feeding bottle to HPLC-DAD, and acquiring results of measurement of anthocyanins in ornamental Chinese flowering apple. The method has the advantages that extraction time is short, less than 2h, the problem that measurement results are affected after the extract is placed overnight for a long period of time is solved, and in terms of computer practice, time is saved greatly and unnecessary waste of reagents is reduced.

Description

technical field [0001] The invention relates to a detection method for flowers, in particular to a detection method for measuring chromosides of ornamental crabapple flowers. Background technique [0002] Anthocyanins are an important class of flavonoid compounds widely present in angiosperms, and it is one of the important material bases for the formation of plant flowers, leaves and fruit color. In nature, anthocyanins are often combined with different types of glycosides and exist in plants in the form of anthocyanins. The research on color-forming substances in plants began in the middle of the 19th century, and many kinds of pigments have been discovered so far, but they can all be classified into the following three categories of pigments: carotenoids, alkaloids and flavonoids (flavonoid). Carotenoids are the general term for carotene and lutein. The conjugated double bonds contained in carotenoids form chromophores, which show yellow, orange, red, purple and other c...

AI Technical Summary

Problems solved by technology

However, in the existing anthocyanin extraction method, the powder made from the leaves is obtained, and the sample formed after adding the anthocyanin extract must be extracted for 72 hours in a refrigerator at 4°C in the dark; during this period, it is shaken every six hours. Collect the supernatant at 24 hours and 48 hours, and then add an equal amount of anthocyanin extract to the sample; after 72 hours, combine the supernatant collected three times, so that the extract must be soaked for a long time (overnight) The problem of inaccurate measurement results due to plant materials

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