Method of measuring anthocyanins in ornamental Chinese flowering apple

A technology of ornamental crabapple and determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as inaccurate determination results, and achieve the effects of short extraction time, time saving and good separation effect

Active Publication Date: 2014-01-08
BEIJING UNIV OF AGRI
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Problems solved by technology

However, in the existing anthocyanin extraction method, the powder made from the leaves is obtained, and the sample formed after adding the anthocyanin extract must be extracted for 72 hours in a refrigerator at 4°C in the dark; during this period, it is shaken every six hours. Collect the supe

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  • Method of measuring anthocyanins in ornamental Chinese flowering apple
  • Method of measuring anthocyanins in ornamental Chinese flowering apple
  • Method of measuring anthocyanins in ornamental Chinese flowering apple

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Embodiment Construction

[0020] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] The embodiment of the present invention provides a method for determining ornamental crabapple anthocyanins, comprising the following steps:

[0022] Step 1, put the ornamental crabapple leaves that have been quick-frozen in liquid nitrogen into a mortar, add quartz sand and insoluble cross-linked polyvinylpyrrolidone (PVPP), add liquid nitrogen and grind them into powder;

[0023] Step 2, transfer the powder into a test tube, add anthocyanin extract, and then obtain it by ultrasonic extraction at 45°C for 60 minutes;

[002...

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Abstract

The invention discloses a method of measuring anthocyanins in ornamental Chinese flowering apple and belongs to the field of flower measurement. The method includes the steps of 1, placing leaves of ornamental Chinese flowering apple which is fast-frozen with liquid nitrogen into a mortar, adding silica sand and insoluble PVPP, and adding liquid hydrogen before grinding to obtain powder; 2, transferring the powder into a test tube, adding anthocyanins extract, performing ultrasonic extraction at 45 DEG C for 60 minutes to obtain extracted anthocyanins liquid; 3, purifying the extracted anthocyanins liquid, centrifuging at the speed of 12000/m for 3 minutes to obtain supernate, and loading the supernate in an Agilent feeding bottle for HPLC detection; 4, feeding a sample, subjecting the Agilent feeding bottle to HPLC-DAD, and acquiring results of measurement of anthocyanins in ornamental Chinese flowering apple. The method has the advantages that extraction time is short, less than 2h, the problem that measurement results are affected after the extract is placed overnight for a long period of time is solved, and in terms of computer practice, time is saved greatly and unnecessary waste of reagents is reduced.

Description

technical field [0001] The invention relates to a detection method for flowers, in particular to a detection method for measuring chromosides of ornamental crabapple flowers. Background technique [0002] Anthocyanins are an important class of flavonoid compounds widely present in angiosperms, and it is one of the important material bases for the formation of plant flowers, leaves and fruit color. In nature, anthocyanins are often combined with different types of glycosides and exist in plants in the form of anthocyanins. The research on color-forming substances in plants began in the middle of the 19th century, and many kinds of pigments have been discovered so far, but they can all be classified into the following three categories of pigments: carotenoids, alkaloids and flavonoids (flavonoid). Carotenoids are the general term for carotene and lutein. The conjugated double bonds contained in carotenoids form chromophores, which show yellow, orange, red, purple and other c...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
Inventor 姚允聪宋婷婷张杰田佶秦晓晓
Owner BEIJING UNIV OF AGRI
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