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Flow-type electric punching device

A technology of flow electroporation and flow electroporation chamber, which is applied in the field of flow electroporation devices to achieve the effect of reducing heat and cathode effect and high-throughput cell processing

Inactive Publication Date: 2014-02-05
ETTA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, although it is relatively simple to design a flow electroporation chamber with parallel plates, it also has many limitations.

Method used

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Examples

Experimental program
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Embodiment 1

[0025] see figure 1 , providing a flow electroporation device, including a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device includes an electrode array 1 and an electrode fixing assembly 2, and the electrode array 2 is fixed on the electrode fixing assembly 1.

[0026] The flow electroporation chamber adopts a circular tubular container, including a cavity 3, an inlet 4, an outlet 5 and an opening 6. There may be multiple inlets 4 and outlets 5, and liquid flows from at least one of them during use. One of the inlets 4 flows in and at least one of the outlets 5 flows out. In this embodiment, the inlet 4 and the outlet 5 are each one, the inlet is at the bottom of the cavity 3, the outlet 4 is at the side of the cavity 3, and the opening 6 is at the bottom of the cavity. The top of body 3. The electrode array 1 is inserted into the cavity 3 through the opening 6 . The flow electroporation chamber can be made of gla...

Embodiment 2

[0033] Others are the same as Embodiment 1, please refer to Image 6 , the second flow electroporation chamber is a cylindrical container, and there are a first interface 7 and a second interface 8 on the second flow electroporation chamber, both of which can be used as sample outlets and inlets.

[0034] The researchers demonstrated that the conditions under which electroporation occurs depend on the type of cell, the type of molecule that is desired to be introduced into the cell, or the type of molecule that is desired to flow out of the cell. For any specific type of cells, there is an optimal treatment condition, called a condition interval. Specifically, a more specific condition can be selected within this condition interval for experiments. This interval of conditions involves the voltage strength, more specifically, the electric field strength applied to the cell, the pulse duration, the number of pulses, and the pulse interval. In the flow electroporation device, th...

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Abstract

The invention discloses a flow-type electric punching device. The flow-type electric punching device comprises a stereoscopic electrode device and a flow-type electric punching chamber, wherein the stereoscopic electrode device comprises an electrode array and an electrode fixing component, the electrode array is fixed on the electrode fixing component, the flow-type electric punching chamber comprises a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening are formed in the cavity, and the electrode array is inserted in the cavity through the opening. By the manner, the flow-type electric punching device can be used for realizing the high-throughput cell treatment, can also work under the condition of relatively low voltage and is capable of avoiding the damage of the influence caused by liquid flowing on cells, reducing the heat caused by voltage pulse and the influence caused by a cathode effect, maintaining a closed aseptic environment and ensuring that the optimal number of times of electric field pulses are applied to the vast majority of cell suspension.

Description

technical field [0001] The invention relates to a device for cell electroporation, in particular to a flow electroporation device. Background technique [0002] Electroporation has been used to insert molecules into animal or plant cells since the early 1970s. The researchers confirmed that when cells are exposed to a transient high-voltage pulsed electric field, the local rupture of the cell membrane caused by the high-voltage electric field will increase the permeability of the cell membrane, thereby forming channels on the surface of the cell membrane, which are called electropores. . These channels exist for a short period of time, but are sufficient for the ingress or egress of macromolecules such as proteins or plasmid DNA. Although cells can tolerate the formation of these channels under high voltage, if the high-voltage pulse voltage is too high, or the duration of the electric field is too long, or the number of high-voltage pulse electric fields is too high, the ...

Claims

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Application Information

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IPC IPC(8): C12M1/42
CPCC12M35/02C12N15/87
Inventor 闫浩吴梦希戴晓兵
Owner ETTA BIOTECH
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