46 results about "Electric field pulse" patented technology

The present invention is related to methods in which an electric field pulse is applied to cells and tissue. Several embodiments of the present invention relate to the application of electric field pulses to cells to regulate the physiology and biophysical properties of various cell types, including terminally differentiated and rapidly dividing cells. Methods of regulating transcription of a gene in a cell, marking a cell for diagnostic or therapeutic procedures, determining cellular tolerance to electroperturbation, selectively electroperturbing a population of cells, reducing proliferation of rapidly dividing cells in a patient, and facilitating entry of a diagnostic or therapeutic agent into a cell's intracellular structures are also provided.

Electroporation electrodes are laminated to a permeable membrane to form an electrode membrane. Such an electrode membrane is useful for a continuous controlled delivery of a therapeutic agent through the skin or mucosa, when placed in direct contact with the skin or mucosa and by application of electric field pulses at specified intervals. An electrode membrane can be assembled such that it incorporates an iontophoretic electrode in the same device. This device can be jointly utilized for electroporation and iontophoretic drug delivery through the skin and mucosal membrane.

An intracellular electro-manipulation apparatus for delivery an electric field pulse to a target of one or more biological cells is provided. The apparatus includes a pulse generator that generates an ultrashort electric field pulse, and a pulse delivery system that directs the ultrashort electric field pulse to the target. The apparatus can include a reflected-signal impeder connected between the pulse generator and the pulse delivery system to impede reflection of a signal to the pulse generator when impedance mismatching between the pulse delivery system and pulse generator occurs. Alternatively, or additionally, the apparatus can include a current limiter connected between the pulse generator and the pulse delivery system to limit current between the pulse generator and the pulse delivery system when a high-conductivity condition occurs in the target.

The invention discloses a method for improving coal seam gas permeability through a high energy acoustoelectric composite technology to mainly solve the problems that the prior art pollutes a coal seam, cannot thoroughly improve the coal seam gas permeability, cannot improve the coal seam gas desorption and seepage and the like. According to the method for improving the coal seam gas permeability through the high energy acoustoelectric composite technology, high electric field pulses and sound waves are combined, the coal seam generates new cracks due to functions of impact wave generation, mechanical vibration and the like, original cracks are expanded, and accordingly coal seam gas transport channels are increased; meanwhile the coal seam gas deabsorption is improved through generated high-temperature effect, cavitation effect and the like and the gas filtration capacity is improved. The method for improving the coal seam gas permeability through the high energy acoustoelectric composite technology comprises a ground drainage well drilling method and a drainage drill hole method parallel to the coal seam. An implementation process comprises the following steps of (1) pulling out devices inside a drill hole; (2) injecting water to the drill hole and drill well through drift tools such as a well gauge, arranging an acoustoelectric composite action device at an appointed position and dividing the coal seam into different sections, wherein the thickness of every section is 30cm; (3) enabling the action device to repeatedly act in the sections; (4) pulling out the devices such as the acoustoelectric composite action device; (5) exhausting and mining the drill hole of the coal seam.

A method of operating a magnetoresistive device is described. The device comprises a ferromagnetic region configured to exhibit magnetic anisotropy and to allow magnetisation thereof to be switched between at least first and second orientations and a gate capacitively coupled to the ferromagnetic region. The method comprises applying an electric field pulse to the ferromagnetic region so as to cause orientation of magnetic anisotropy to change for switching magnetisation between the first and second orientations.

A method of treating tumor tissue of an individual is provided. The method is effected by applying to cells of the tumor tissue electrical field pulses having a strength, a repetition frequency and a pulse width selected capable of inducing endocytosis mediated cell death thereby treating the tumor tissue.

The invention relates to a method for treating products, which may contain cellular material of eukaryotic and/or prokaryotic origin and in particular micro-organisms, by bringing the product in device comprising two electrodes connected to an electronic circuit such that in said device and in said product a pulsating electrical field is created, characterized by the rise time of each imposed voltage pulse which is less than the electronic relaxation time of the product. Preferably the rising edge of each cycle starts within the relaxation time of the product and even more preferably each electrical field pulse has a duration shorter than the relaxation time of the product. Dependent on the type of product and the types of micro-organisms contained in the product, the maximum fieldstrength of each pulse, the repetition frequency and the number of cycles in the treatment are selected such that the target micro-organisms and/or spores are functionally inactivated leading to a shelf stable, microbiologically safe product.

Provided is an electro-microneedle integrated body in which a dissolving microneedle and an electrode for electroporation are integrated into one, which enables a focused and efficient intracutaneous gene release for percutaneous gene delivery and intracellular gene delivery occurring in one in-situ treatment site The electro-microneedle integrated body according to the present invention includes an electrode for electroporation which is contacted with skin of a human body to apply an electric field pulse, including a base part and a plurality of electrode parts protruding from the base part, and a microneedle adhered to each electrode part and inserted into the skin of a human body, including a biocompatible and biodegradable viscous material and a genetic material, wherein the microneedle degrades within the skin, and the electric field pulse is applied through the electrode for electroporation in a site in which the microneedle is inserted.

When ink is ejected from any one of a plurality of pressure chambers, an electric field pulse, which corresponds to an electric field pulse to be applied to a partition wall of the pressure chamber which is to eject the ink, and has at least one square wave, which is in a direction opposite to that of the electric field pulse and has a pulse width corresponding to the electric field pulse, is applied to a partition wall adjacent to the partition wall of the pressure chamber which is to eject the ink.

The invention discloses a flow-type electric punching device. The flow-type electric punching device comprises a stereoscopic electrode device and a flow-type electric punching chamber, wherein the stereoscopic electrode device comprises an electrode array and an electrode fixing component, the electrode array is fixed on the electrode fixing component, the flow-type electric punching chamber comprises a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening are formed in the cavity, and the electrode array is inserted in the cavity through the opening. By the manner, the flow-type electric punching device can be used for realizing the high-throughput cell treatment, can also work under the condition of relatively low voltage and is capable of avoiding the damage of the influence caused by liquid flowing on cells, reducing the heat caused by voltage pulse and the influence caused by a cathode effect, maintaining a closed aseptic environment and ensuring that the optimal number of times of electric field pulses are applied to the vast majority of cell suspension.

The invention provides a multi-ferric heterojunction and a modulation method for achieving magnetic torque nonvolatile orientation of magnetic film based on the multi-ferric heterojunction, and belongs to the technical field of electronic material. The multi-ferric heterojunction uses cheap polycrystal PZT ceramic containing defect dipoles as a piezoelectric substrate, an elargol is coated on one side thereof for being used as an electrode, the other side is polished and then plated or glued with a magnetic film having magnetostriction features; the polycrystal PZT ceramic substrate containing the defect dipoles is obtained by polarizing and aging the polycrystal PZT ceramic substrate after being acceptor doped. By applying a specific voltage pulse to the multi-ferric heterojunction, three nonvolatile transformation states can be generated in the magnetic film after removing the externally applied voltage, thus achieving the stable nonvolatile magnetic torque modulation effect. The modulation method is simple and convenient in operation, easy to be achieved, and good in modulation effect; the modulation method has a wide application range in the field of nonvolatile electric field pulse modulation magnetic devices.

An electroporation cuvette is constructed with electroporation electrodes arranged in non-parallel relation to form a gap whose width varies with the location within the cuvette, plus a pair of positioning electrodes that are arranged to cause electrophoretic migration of biological cells within the cuvette according to cell size. Once the cells, suspended in a solution of the impregnant, are distributed in the cuvette by the positioning electrodes, electric field pulses are generated by the non-parallel electroporation electrodes. Because of their distribution in the cuvette, the various cells will experience voltage differentials across their widths that approach uniformity regardless of cell diameter, since the larger cells will be positioned at locations where the gap between the electrodes is greater and the smaller cells at locations where the gap is relatively small while the voltage drop across the entire gap is uniform along the length of the cell. The voltage differential across the width of the cell is thus roughly paired with the cell diameter, and this reduces the disparity in voltage differential that cells of different sizes would otherwise experience with parallel electrodes.

The invention relates to a GIS switch fault diagnosis method based on measurement of a radiating electric field of VFTO (Very Fast Transient Overvoltage) generated by switching operation. The method comprises the following steps that (1) the radiating electric field of the VFTO generated by switching operation in a GIS is measured; (2) the step (1) is repeated to measure the radiating electric field after multiple times of switching operation, and accumulated measuring data is summarized to obtain a standard radiating electric field oscillogram of the switching operation when the GIS is in a normal state; and (3) the state of the GIS is monitored, and when the pulse waveform of the radiating electric field of the VFTO generated by corresponding switching operation is different from the standard radiating electric field oscillogram obtained in the step (2), it can be determined that a switch in the GIS has an insulation fault. Thus, the electric-field pulses measured by the method are proved to be highly effective and accurate according to repeated experiments.

The invention relates to a sterilisation method that comprises heating the liquid with electric field pulses having a frequency higher that 1 MHz at a rate higher than 28 DEG C per second to a processing temperature T between 20 and 66 DEG C, and depending on the value of the processing temperature T, exposing the liquid to a pulsed alternating electric field immediately or shortly after heating the liquid.

A pulsatile, positive-displacement mechanical circulatory support pump which can be used for assistance or replacement of one or both ventricles. The pump includes a plurality of contractile elements radiating outward from an apex of a compliance chamber, the elements being incorporated in and/or in contact with at least a portion of an outer surface of the compliance chamber. These contractile elements include an electroactive dielectric elastomer or an ion exchange membrane metallic composite. Application of electric field pulses from an implantable electrical energy source such as a pacemaker will cause the contractile elements to compress and expand the compliance chamber.

The invention provides a device for measuring electromagnetic pulses generated by spacecraft electrostatic discharge. The device comprises a monopole antenna and an electrical control unit. The monopole antenna transmits sensed transient electric field pulse signals to the electrical control unit through a cable to be acquired and processed by the electrical control unit and effective data are stored and then transmitted to the ground to be analyzed. Compared with the devices in the prior art, on-orbit in-situ measurement of the electromagnetic pulses generated by spacecraft electrostatic discharge can be realized by adopting the mode of combination of the monopole antenna and the electrical control unit; and the device for measuring the electromagnetic pulses generated by on-orbit spacecraft electrostatic discharge and the method thereof can realize measurement of narrow pulse width and high-amplitude discharge pulses, and the whole device has the characteristics of small data volume, low power consumption and low weight.