Application of a strain of Bacillus amyloliquefaciens and its agent in the prevention and treatment of olive fruit rot
A technology for dissolving starch spores and rot diseases, applied in the field of biology, can solve the problems of non-pollution prevention and control technology not involved, no effective control methods, etc., and achieve the effect of preventing and treating rot diseases.
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[0015] The present invention will be described in detail below in conjunction with specific embodiments.
[0016] 1. Isolation, purification and preservation of endophytic bacteria
[0017] The healthy walnut root system was collected from the place where walnut root rot occurred in Chaotian District, Guangyuan City, Sichuan Province, and brought back to the laboratory for isolation. The collected samples were rinsed with sterile water, weighed 1.0 g of root tissue, disinfected with 70% alcohol for 30 seconds, washed with sterile water for 3 times, then disinfected with 4% NaClO for 3 minutes, washed with sterile water for 3 times, absorbed 100 μl of washing solution for the last time to smear on NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000ml distilled water, pH7.0, after mixing and dispensing, autoclave at 121°C for 30min), in the dark at 28°C Cultivate for 24 hours and use it as a control for surface disinfection to check whether the ...
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