Bacillus amyloliquefaciens and application of bacterial agent thereof in prevention and control of alternaria tenuissima

A technology for dissolving starch spores and rot diseases, applied in the field of biology, can solve the problems of non-pollution prevention and control technology not involved, no effective control methods, etc., and achieve the effect of preventing and treating rot diseases.

Inactive Publication Date: 2014-02-05
SICHUAN AGRI UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective control method, especially the non-pollution control technology has not involved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus amyloliquefaciens and application of bacterial agent thereof in prevention and control of alternaria tenuissima
  • Bacillus amyloliquefaciens and application of bacterial agent thereof in prevention and control of alternaria tenuissima
  • Bacillus amyloliquefaciens and application of bacterial agent thereof in prevention and control of alternaria tenuissima

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific embodiments.

[0016] 1. Isolation, purification and preservation of endophytic bacteria

[0017] The healthy walnut root system was collected from the place where walnut root rot occurred in Chaotian District, Guangyuan City, Sichuan Province, and brought back to the laboratory for isolation. The collected samples were rinsed with sterile water, weighed 1.0 g of root tissue, disinfected with 70% alcohol for 30 seconds, washed with sterile water for 3 times, then disinfected with 4% NaClO for 3 minutes, washed with sterile water for 3 times, absorbed 100 μl of washing solution for the last time to smear on NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000ml distilled water, pH7.0, after mixing and dispensing, autoclave at 121°C for 30min), in the dark at 28°C Cultivate for 24 hours and use it as a control for surface disinfection to check whether the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses bacillus amyloliquefaciens and application of a bacterial agent thereof in prevention and control of alternaria tenuissima. The strain has a classification name of bacillus amyloliquefaciens, the complete name of the strain is bacillus amyloliquefaciens Ba4, and the strain is collected in China General Microbiological Culture Collection Center in 3# No.1 courtyard on Beichen west road in Chaoyang district in Beijing, China on 12, September in 2013 and has a collection number of CGMCC No.8177. According to the bacillus amyloliquefaciens Ba4 provided in the invention, the alternaria tenuissima can be effectively prevented and controlled.

Description

technical field [0001] The invention relates to the field of biology, in particular to the application of a strain of bacillus amyloliquefaciens and its agent in preventing and treating olive fruit rot. Background technique [0002] Olive fruit rot (Alternaria tenuissima) is a new disease in olive producing areas. It mainly damages fruits and leaves, and causes fruit and leaf fall. After the fruit is infected, it is a small black spot at first, and gradually expands, and the spot can be combined into a large spot, and the fruit falls off and rots. At present, there is no effective control method, especially the non-pollution control technology is not involved. Olive fruit is an important raw material for olive oil production. Therefore, the use of pollution-free and environmentally friendly measures is the first choice for the prevention and treatment of this disease. [0003] Therefore, there are defects in the prior art and need to be improved. Contents of the inventio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 朱天辉朱涵明月李姝江张静刘洋韩珊谯天敏张丽娜
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap