Method for separating and extracting pig placentin

Abstract

The invention relates to a method for separating and extracting pig placentin, and belongs to the field of research and development of health-care foods. The method takes the pig placenta as a raw material, and comprises the steps of carrying out high-speed tissue homogenization, low-temperature and low-frequency ultrasonic-assisted extraction and high-speed refrigerated centrifugation on the pig placenta to obtain a coarse extract, wherein the content of soluble proteins is 2-4mg / mL; carrying out ultrafiltration by adopting a 10KDa ultrafiltration membrane to obtain a pig placentin liquid with the low molecular weight, wherein the content of polypeptide is 600-1000mu g / mL; carrying out microwave vacuum freeze-drying to obtain a solid pig placentin product. The pig placentin produced by using the method has functions of nutrition supply and health care; low-frequency ultrasonic waves (28-45kHz) are used for extracting in an auxiliary way, so that the extraction rate in the method is 10-15% higher than that in the method without ultrasonic assistance; the microwave vacuum freeze-drying technology is adopted, the drying time of the method is shortened by 30-40% compared with that of the common vacuum freeze-drying; furthermore, after the method is adopted, the loss of the nutritive value of the pig placentin is small, and the separating efficiency and the extracting efficiency of the pig placentin can be improved.

Description

technical field

[0001] A method for separating and extracting pig placenta, which uses pig placenta as raw material, undergoes high-speed tissue homogenization, low-temperature and low-frequency ultrasonic-assisted extraction, high-speed refrigerated centrifugation and ultrafiltration with a 10KDa ultrafiltration membrane to obtain a low-molecular-weight pig placenta solution, The solid porcine placenta product is obtained through microwave vacuum freeze-drying, which involves a low-temperature ultrasonic extraction process and a microwave vacuum freeze-drying process, and belongs to the research and development field of health food. Background technique

[0002] Porcine placenta refers to the use of biotechnology (low temperature extraction, freezing technology, etc.), through cell crushing, low temperature dissolution extraction, ultrafiltration, dialysis and other steps to purify, or after freeze-drying small molecular polypeptide substances, is a kind of Pure nat...