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A kind of separation and extraction method of pig placenta

A technology for pig placenta and its extraction method, which is applied in the field of separation and extraction of pig placenta, can solve the problems of loss of pig placenta bioactivity, long processing steps and long time, etc., achieves maintenance of bioactivity and nutritional value, less loss of nutritional value, The effect of shortening the drying time

Inactive Publication Date: 2015-08-26
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, the above separation, extraction and processing of pig placenta more or less utilize high temperature, high-concentration salt treatment, strong acid treatment, and strong alkali treatment, which will easily cause the loss of the biological activity of pig placenta, and the processing steps and time are too short. longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: 28kHz low-frequency ultrasonic-assisted efficient separation and extraction of porcine placenta

[0028] Take the pig placenta that has been born naturally and passed the immunological test. After washing the surface with clean water without blood stains and other debris, remove the blood vessels and fascia, divide it into 2cm×2cm small pieces, wash it again with clean water until there is no blood stain on the surface, and place it at ultra-low temperature Freeze at -65°C in the refrigerator for later use. When in use, take the frozen pig placenta raw material, thaw it at room temperature, cut it into pieces with scissors to a size of 0.5cm×0.5cm, and wash it again until there is no blood stain. Adjust the material-to-liquid ratio (pig placenta raw material: deionized water w / w) to 2:4, homogenize in a 10000rpm tissue homogenizer until the homogenate is in a state of natural flow, and keep the temperature of the homogenate not exceeding 40°C.

[0029] A ce...

Embodiment 2

[0032] Example 2: 45kHz low-frequency ultrasonic-assisted efficient separation and extraction of porcine placenta

[0033] Take the pig placenta that has been born naturally and passed the immunological test. After washing the surface with clean water without blood stains and other debris, remove the blood vessels and fascia, divide it into 2cm×2cm small pieces, wash it again with clean water until the surface is free of blood stains, and place it in ultra-low temperature Freeze at -65°C in the refrigerator for later use. When in use, take the frozen pig placenta raw material, thaw it at room temperature, cut it into pieces with scissors to a size of 0.5cm×0.5cm, and wash it again until there is no blood stain. Adjust the material-to-liquid ratio (pig placenta raw material: deionized water w / w) to 2:5, homogenize in a 10000rpm tissue homogenizer until the homogenate is in a natural flow state, and keep the temperature of the homogenate not exceeding 40°C.

[0034] A certain ...

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PUM

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Abstract

The invention relates to a method for separating and extracting pig placentin, and belongs to the field of research and development of health-care foods. The method takes the pig placenta as a raw material, and comprises the steps of carrying out high-speed tissue homogenization, low-temperature and low-frequency ultrasonic-assisted extraction and high-speed refrigerated centrifugation on the pig placenta to obtain a coarse extract, wherein the content of soluble proteins is 2-4mg / mL; carrying out ultrafiltration by adopting a 10KDa ultrafiltration membrane to obtain a pig placentin liquid with the low molecular weight, wherein the content of polypeptide is 600-1000mu g / mL; carrying out microwave vacuum freeze-drying to obtain a solid pig placentin product. The pig placentin produced by using the method has functions of nutrition supply and health care; low-frequency ultrasonic waves (28-45kHz) are used for extracting in an auxiliary way, so that the extraction rate in the method is 10-15% higher than that in the method without ultrasonic assistance; the microwave vacuum freeze-drying technology is adopted, the drying time of the method is shortened by 30-40% compared with that of the common vacuum freeze-drying; furthermore, after the method is adopted, the loss of the nutritive value of the pig placentin is small, and the separating efficiency and the extracting efficiency of the pig placentin can be improved.

Description

technical field [0001] A method for separating and extracting pig placenta, which uses pig placenta as raw material, undergoes high-speed tissue homogenization, low-temperature and low-frequency ultrasonic-assisted extraction, high-speed refrigerated centrifugation and ultrafiltration with a 10KDa ultrafiltration membrane to obtain a low-molecular-weight pig placenta solution, The solid porcine placenta product is obtained through microwave vacuum freeze-drying, which involves a low-temperature ultrasonic extraction process and a microwave vacuum freeze-drying process, and belongs to the research and development field of health food. Background technique [0002] Porcine placenta refers to the use of biotechnology (low temperature extraction, freezing technology, etc.), through cell crushing, low temperature dissolution extraction, ultrafiltration, dialysis and other steps to purify, or after freeze-drying small molecular polypeptide substances, is a kind of Pure nat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/50A23L1/29A61P37/04A23L33/00
Inventor 张慜唐文林陈志雄刘亚萍陈世豪
Owner JIANGNAN UNIV
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