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Euphorbiaceae plant active substance for inhibiting cancer and/or tumor cell growth and application thereof

A technology of tumor cells and active substances, applied in the field of animal health care with active substances of Euphorbiaceae plants, can solve the problems of tumor recurrence or metastasis, cost considerations, etc. Effect

Inactive Publication Date: 2018-12-21
SCHWEITZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Almost all of the anti-cancer and / or tumor drugs or health foods currently on the market are developed for the purpose of treating human cancers; if these expensive anti-cancer and / or tumor drugs or health foods are applied to animals, There may be cost considerations; therefore, surgery is still the main method for treating animal tumors at this stage, but there is still a high probability of tumor recurrence or metastasis after surgery

Method used

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  • Euphorbiaceae plant active substance for inhibiting cancer and/or tumor cell growth and application thereof
  • Euphorbiaceae plant active substance for inhibiting cancer and/or tumor cell growth and application thereof
  • Euphorbiaceae plant active substance for inhibiting cancer and/or tumor cell growth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment one Euphorbiaceae plant active substance

[0046] 1. Active substances extracted from Ethanol

[0047] Euphorbia neriifolia was collected from Yilan, Taiwan. Firstly, wash the stems and leaves of the collected vajras with water, let them dry, weigh them and cut them into slices, then put the stems and leaves of vajras in a brown glass bottle that can be protected from light, and add 95% ethanol to make them dry. The stems and leaves of the diamondback are completely soaked in ethanol, and stand at room temperature for extraction. When the ethanol turns from transparent and colorless to dark green or dark brown (about 3 to 10 days), the ethanol extract is collected, and then concentrated under reduced pressure to remove Ethanol, in order to obtain the active substance extracted from Ethanol.

[0048] 2. Active substances extracted by n-hexane (Hexane)

[0049] Get the active substance 10g of above-mentioned vajra ethanol extraction and be...

Embodiment 2

[0050] Example 2 Analysis of Euphorbiaceae Plant Active Substances Inhibiting Tumor Cell Proliferation

[0051] 1. Test method

[0052] In this embodiment, canine breast tumor cell lines, CMT-1 (canine mammary tumor-1) cell line and MPG cell line are used as test objects. CMT-1 cells or MPG cells were seeded in 96-well flat-bottom cell culture plates at a density of 3,000-5,000 cells per well, and cultured in DMEM containing 5-10% fetal bovine serum and 1% antibacterial and antifungal solution liquid culture at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours. After confirming that the cells are attached to the bottom of the culture dish, replace the original cell culture medium with DMEM cell culture medium (2ml / well) containing 0.5% fetal bovine serum and 1% antibacterial and antifungal liquid. Add different concentrations (2.5 μg / ml, 5 μg / ml, 10 μg / ml, 20 μg / ml) of the adamantium ethanol-extracted active substance or adamantine-n-hexane extracted active substance obt...

Embodiment 3

[0060] Example 3 Analysis of Effects of Euphorbiaceae Plant Active Substances on Tumor Cell Survival

[0061] 1. Analysis of cell viability

[0062] CMT-1 cells or MPG cells were added to each well at 1x10 5 -4x10 5 The density of the single cell was inoculated in a 6-well flat-bottomed cell culture dish, and cultured in DMEM cell culture medium containing 5-10% fetal bovine serum and 1% antibacterial and antifungal liquid, at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours. After confirming that the cells are attached to the bottom of the culture dish, replace the original cell culture medium with DMEM cell culture medium (2ml / well) containing 0.5% fetal bovine serum and 1% antibacterial and antifungal liquid. And add the active substance (10 μg / ml, 20 μg / ml) or the active substance (5 μg / ml, 10 μg / ml) of the adamantine ethanol extraction obtained by Example 1 or the active substance extracted by adamantine n-hexane in different concentrations. And on the third day o...

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Abstract

The present invention relates to an euphorbiaceae plant active substance for restraining growth of cancer and / or tumor and application thereof. The euphorbiaceae plant active substance is extracted from the euphorbiaceae plant by alcohol and furthermore can be extracted by n-hexane. The invention further relates to use of the euphorbiaceae plant active substance in preparing a composition for restraining growth of the cancer and / or tumor cell.

Description

technical field [0001] The present invention relates to the application of active substances of Euphorbiaceae plants (Euphorbiaceae) in the field of animal health care, in particular to the application of active substances of Euphorbiaceae plants in inhibiting and treating cancer and / or tumors. Background technique [0002] In recent years, the application of Chinese herbal medicine in medicine has gradually been paid attention to; compared with chemically synthesized medicines, the extracts of these natural plants have fewer side effects and are relatively safe. It has been pointed out in literature that the extracts of Pittosporaceae and Euphorbiaceae have the ability to inhibit human cytomegalovirus (Human Cytomegalovirus, HCMV) (Semple et al., 1998); Plant extracts from different genera of the same family of Halberdaceae can inhibit the hepatitis B virus (Hepatitis B virus, HBV) (Unander et al., 1995) or have the ability to inhibit the second type of herpes virus (Herpes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/47A61P35/04A61P35/00A61K127/00A61K135/00
Inventor 郭村勇杨滢臻廖泰庆林辰栖
Owner SCHWEITZER
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