Nucleic acid amplification device and nucleic acid analysis device

A nucleic acid amplification device and nucleic acid analysis technology, applied in the field of nucleic acid analysis devices to achieve the effect of achieving temperature uniformity

Active Publication Date: 2016-04-27
HITACHI HIGH-TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, there are cases where a plurality of temperature control modules that can individually control the temperature are mounted on one device

Method used

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  • Nucleic acid amplification device and nucleic acid analysis device
  • Nucleic acid amplification device and nucleic acid analysis device
  • Nucleic acid amplification device and nucleic acid analysis device

Examples

Experimental program
Comparison scheme
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specific example 1

[0070] image 3 A specific example of a nucleic acid amplification device that corrects the absolute values ​​of temperatures of a plurality of temperature control modules 1 using one temperature calibration sample is shown. image 3 The device configuration shown is for the figure 2 The configuration of the device shown is a configuration shown in more detail.

[0071] In the case of this embodiment example, four temperature control modules 1 capable of individually controlling the temperature are arranged along the outer edge of the disc-shaped rotating disk 22 . The rotary disk 22 here corresponds to the base of the embodiment example 1. As shown in FIG. The rotary disk 22 is made of a material excellent in heat insulation. Therefore, the mutual interference of temperature among multiple temperature control modules can be ignored.

[0072] The rotary disk 22 is fixed to an unillustrated rotary shaft, and can freely rotate both clockwise and counterclockwise as indicate...

specific example 2

[0082] Figure 4 Another device configuration example of a nucleic acid amplification device that corrects the temperature of the temperature control module 1 using one type of temperature calibration sample is shown. Figure 4 The device configuration shown is also for figure 2 The shown device configuration is a device configuration shown in more detail.

[0083] exist image 3 In the case of the device configuration shown, the number of real-time fluorescence measurement units 3 is reduced relative to the number of temperature control modules 1 . Therefore, either one of the base on which the temperature control module 1 is placed and the real-time fluorescence measurement unit 3 is fixed, and the rotation of the other is controlled.

[0084] However, in the case where the temperature control module 1 and the real-time fluorescence measurement part 3 correspond one-to-one and there are multiple temperature control modules 1 and real-time fluorescence measurement parts 3...

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Abstract

According to a conventional technique, when a calibrated temperature measuring probe is used for correcting the temperature absolute values of individually temperature-controllable thermal control blocks, a temperature difference of a maximum of 0.5° C. remains between the thermal control blocks. According to the present invention, the melting temperature of a temperature calibration sample housed in a reaction vessel corresponding to each of the temperature control blocks is measured as a measured melting temperature. The measured melting temperature corresponding to each of the thermal control blocks and the reference melting temperature of the temperature calibration sample are compared, and the temperature absolute value of each of the thermal control blocks is corrected based on respective difference values.

Description

technical field [0001] The present invention relates to a nucleic acid amplification device equipped with a plurality of temperature control modules capable of individually controlling temperatures, and a nucleic acid analysis device using the nucleic acid amplification device as part of the device. Background technique [0002] The temperature control module needs to control the absolute value of its temperature correctly at the target temperature. Conventionally, in the measurement of the absolute value of the temperature of the temperature control module, the following method is used: when the measured temperature is different from the target temperature using a calibrated temperature measurement probe, correction is made so that the measured temperature matches the target temperature. . In general, the limit accuracy of temperature correction using a calibrated temperature measurement probe is ±0.25°C. [0003] However, there are cases where a plurality of temperature ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCB01L7/52B01L2200/148B01L2300/024B01L2300/0829B01L2300/1822
Inventor 前田耕史杉山千枝庄司义之石泽雅人佐野稔
Owner HITACHI HIGH-TECH CORP
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