Inducing medium and method for improving survival rate of butterfly orchid pedicel

A technique for inducing culture medium and culture medium, applied in cell culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of low survival rate of pedicels, large damage to explants, incomplete disinfection, etc., and achieve the purpose of inhibiting browning The effects of chemicalization, improvement of survival rate, and shortening of germination time

Inactive Publication Date: 2014-04-02
TODAYS LANDSCAPING BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the process of using pedicel axillary buds as explants to induce the cultivation of Phalaenopsis, there are often problems such as incomplete disinfection during the initial induction culture, great damage to the explants during sterilization,

Method used

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  • Inducing medium and method for improving survival rate of butterfly orchid pedicel
  • Inducing medium and method for improving survival rate of butterfly orchid pedicel
  • Inducing medium and method for improving survival rate of butterfly orchid pedicel

Examples

Experimental program
Comparison scheme
Effect test

Example

[0027] Examples 1-7

[0028] 1) Preparation of the induction medium used in Examples 1-7

[0029] The induction medium of Examples 1-7 is prepared by adding agar, white sugar, activated carbon, coconut juice, carbenicillin, 6-benzylamino adenine, and naphthalene acetic acid to 1 / 2MS medium, agar, white sugar, Refer to Table 1 for the concentrations of activated carbon, coconut water, carbenicillin, 6-benzylamino adenine, and naphthalene acetic acid in the induction medium of Examples 1-7. The induction medium numbers 1 to 7 in Table 1 correspond to the induction medium of Examples 1 to 7, respectively. After preparing the induction medium of Examples 1-7, they were placed in bottles 1-7, respectively. The formula of 1 / 2MS medium used is shown in Table 2 below. The dosage mentioned in Table 2 refers to the dosage when preparing 1 liter of 1 / 2MS medium, and the amount of macro elements in 1 / 2MS medium It is half the amount of macronutrients in standard MS medium.

[0030] Table 1 C...

Example

[0040] Comparative example 1~7

[0041] Comparative Examples 1-7 are basically the same as Examples 1-7, respectively, with the difference that in Step 2), Comparative Examples 1-7 do not have the step of immersing the pedicels in a 200 mg / L carbenicillin solution.

[0042] The pedicels inoculated in the induction medium of Examples 1-7 were cultured for about 14-18 days, and it was found that the axillary buds germinated and expanded. After the pedicels inoculated in the induction medium of Examples 1-7 and Comparative Examples 1-7 were induced and cultured for 30 days, the pollution rate and germination rate of the Phalaenopsis stalk in each culture flask were counted. The experimental data are shown in Table 3.

[0043] Table 3 Experimental data after 30 days of cultivation

[0044]

[0045] In Table 3, the germination rate after deducting the contaminated bottle, the germination bud number / sterile bud number×100%

[0046] In Table 3, the comprehensive evaluation y=[(1-pollution rat...

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Abstract

The invention provides an inducing medium and a method for improving a survival rate of a butterfly orchid pedicel. The inducing medium is prepared by adding agar, white granulated sugar, 6-benzylaminopurine, naphthylacetic acid, carbenicillin, coconut milk and activated carbon in a 1/2 MS (Murashige-Skoog) culture medium. The inducing medium comprises the following ingredients at concentration: 7-9 g/L of agar, 15-30 g/L of white granulated sugar, 0-50 mg/L of carbenicillin, 1.5-3 mg/L of 6-benzylaminopurine, 0.2-0.5 mg/L of naphthylacetic acid, 0-100 g/L of coconut milk, and 1-3 g/L of activated carbon. The inducing medium can be used for inducing the butterfly orchid pedicel. The method for improving the survival rate of the butterfly orchid pedicel is low in cost, and low in the sterilizing contamination rate, and can effectively improves the survival rate of the sterilized explant.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture industrialized seedling cultivation, and in particular relates to a method for effectively improving the survival rate and induction effect of the Phalaenopsis pedicels when using the Phalaenopsis pedicels as explants for primary culture. Background technique [0002] Phalaenopsis B1.spp. is a tropical aerial orchid, which has large flowers, long flowering period, gorgeous flower color, rich color, beautiful and unique flower shape, dancing like a butterfly, hence the name. Known as the "Queen of Orchids" among tropical orchids, it is one of the most popular catayas in recent years. [0003] Phalaenopsis is a single-stem aerial orchid, and the plants rarely develop side buds, and the seeds are extremely difficult to germinate. Its conventional propagation is very slow, so tissue culture is often used for rapid propagation. Disinfection of explants is the first step in tissue rapid pr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008C12N5/0025A01H4/002
Inventor 陈永得
Owner TODAYS LANDSCAPING BIOLOGICAL TECH
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