A method for decolorizing silkworm chrysalis proteolysis of blood pressure-lowering peptide

A technology of proteolysis and antihypertensive peptides, which is applied in the field of decolorization of silkworm chrysalis proteolytic antihypertensive peptides, can solve problems such as adverse clinical reactions, and achieve low equipment requirements, good selectivity, anion and cation exchange functions, and simple decolorization methods Effect

Active Publication Date: 2015-12-09
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Angiotensin-inhibiting drugs are one of the main antihypertensive drugs, but some synthetic angiotensin-inhibiting drugs have adverse clinical reactions, making angiotensin-inhibiting peptides in natural foods the main research and development target at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of silkworm chrysalis protein enzymatic hydrolysis blood pressure-lowering peptide liquid: dissolve 40g silkworm chrysalis protein powder into 2L water to form silkworm chrysalis protein water-soluble, add trypsin as hydrolytic enzyme, the addition amount of trypsin is 1% of silkworm chrysalis protein quality, control the pH at Under the environment of 8.0 for enzymatic hydrolysis for 4 hours, use NaOH solution to maintain a constant pH value, and inactivate the enzyme to obtain silkworm chrysalis proteolytic blood pressure lowering peptide solution. It has been determined that the angiotensin inhibitory activity is 90.1%, and the protein content of the enzymatic solution is 10 mg·L -1 , the detected amount of angiotensin inhibitory activity protein was 200 μg.

[0022] 2. Decolorization of silkworm chrysalis protease hydrolyzed hypotensive peptide solution

[0023] The pH value of the prepared silkworm chrysalis proteolysis blood pressure lowering pepti...

Embodiment 2

[0025] 1. The preparation of the antihypertensive peptide liquid hydrolyzed by silkworm chrysalis proteolysis is the same as that in Example 1.

[0026] 2. Decolorization of silkworm chrysalis protease hydrolyzed hypotensive peptide solution

[0027] The pH value of the silkworm chrysalis proteolytic blood pressure-lowering peptide solution prepared in Example 1 was adjusted to 7.5, and the mass ratio of bentonite, activated clay and alkaline calcium-based bentonite in the decolorizing agent was 1:0.8:1. The decolorizing agent and silkworm chrysalis protease The mass-to-volume ratio of the antihypertensive peptide solution is 30g / L. During ultrasonic oscillation, adjust the ultrasonic initial temperature to 40°C, ultrasonic power to 35W, and decolorize for 25 minutes; centrifuge at a speed of 4000r·min -1 After centrifugation for 20 minutes, the obtained supernatant was decolorized silkworm chrysalis protein hypotensive enzymatic hydrolyzate. After testing, the final decoloriz...

Embodiment 3

[0029] 1. The preparation of the antihypertensive peptide liquid hydrolyzed by silkworm chrysalis proteolysis is the same as that in Example 1.

[0030] 2. Decolorization of silkworm chrysalis protease hydrolyzed hypotensive peptide solution

[0031] The pH value of the silkworm chrysalis proteolytic blood pressure-lowering peptide solution prepared in Example 1 was adjusted to 7.0, and the mass ratio of bentonite, activated clay and alkaline calcium-based bentonite in the decolorizing agent was 1:1:1. The decolorizing agent and silkworm chrysalis protease The mass-to-volume ratio of the antihypertensive peptide solution is 35g / L. During ultrasonic oscillation, adjust the ultrasonic initial temperature to 45°C, ultrasonic power 40W, and decolorize for 20 minutes; centrifugal separation speed 4000r·min -1 After centrifugation for 20 minutes, the obtained supernatant was decolorized silkworm chrysalis protein hypotensive enzymatic hydrolyzate. After testing, the final decoloriza...

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Abstract

The invention discloses a decoloration method of silkworm chrysalis proteolysis antihypertensive peptide, which comprises the steps of adjusting the pH value of the silkworm chrysalis proteolysis antihypertensive peptide to 6-9; adding a mixture of bentonite, activated clay and alkaline calcium bentonite as a decoloration agent, wherein the mass volume ratio of the decoloration agent to the silkworm chrysalis proteolysis antihypertensive peptide is 10-45g / L; performing ultrasonic oscillation decoloration for 15-30 minutes at 30-45 DEG C; performing centrifugal separation, wherein the obtained supernate is the silkworm chrysalis proteolysis antihypertensive peptide after decoloration treatment. According to the method disclosed by the invention, by adopting the mixture of bentonite, activated clay and alkaline calcium bentonite as the decoloration agent, a relatively high decoloration rate is guaranteed, and the antihypertensive inhibition activity (ACE inhibition activity) is effectively maintained; moreover, the method is simple and easy to operate, has mild treatment processes, and can be widely applied to the production processes of the silkworm chrysalis polypeptide and other active peptides.

Description

technical field [0001] The invention relates to the technical field of food decolorization, in particular to a decolorization method for enzymatically hydrolyzing blood pressure-lowering peptides from silkworm chrysalis. Background technique [0002] Silkworm chrysalis (Silkwormpupa) is the chrysalis of the Bombyx moth of the silk moth family insect, and is a large by-product of the silk industry. According to the statistics of the Ministry of Commerce of the People's Republic of China, in 2009 the output of dried silkworm cocoons, the raw material of silk, reached 782,100 tons. Half of the dry weight of silkworm cocoons is silkworm chrysalis. Due to the lack of deep processing technology, a large number of silkworm chrysalis are used as feed and fertilizer, and the resource utilization rate and added value are low. The silkworm chrysalis protein accounts for 45%-50% of the dry silkworm chrysalis mass. It has been determined that the silkworm chrysalis protein contains 18 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C12P21/06
Inventor 赵钟兴韦藤幼廖丹葵孙建华童张法
Owner GUANGXI UNIV
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