Tobacco squalene synthase?protein, tobacco squalene synthase?gene and application

A technology of ene synthase protein and tobacco shark, applied in application, genetic engineering, plant gene improvement, etc., can solve the problems of reducing tobacco sterol content and achieve the effect of reducing tobacco sterol content

Active Publication Date: 2014-05-28
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the structure of sterols all contain hydroxyl groups, the tetracyclopentene[α]phenanthrene ring structure of its parent can form fused-ring aromatic hydrocarbons during pyrolysis. Therefore, sterols in tobacco are potential substances that

Method used

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  • Tobacco squalene synthase?protein, tobacco squalene synthase?gene and application
  • Tobacco squalene synthase?protein, tobacco squalene synthase?gene and application
  • Tobacco squalene synthase?protein, tobacco squalene synthase?gene and application

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Embodiment Construction

[0024] A tobacco squalene synthase protein, the amino acid sequence of which is shown in SEQ ID NO:1.

[0025] A tobacco squalene synthase gene, encoding the gene of the tobacco squalene synthase protein described in claim 1.

[0026] A tobacco squalene synthase gene, the base sequence of which is shown in SEQ ID NO:2.

[0027] The specific nucleic acid fragment of the tobacco squalene synthase gene is the 577th-1092nd nucleotide sequence of SEQ ID NO: 2 in the sequence listing.

[0028] The application of the tobacco squalene synthase gene in reducing the content of tobacco sterol. Interfering, silencing, or knocking out the tobacco squalene synthase by transgenic technology or transient expression technology to obtain transformed tobacco plants with reduced sterol content. The virus-induced silencing vector or RNAi interference vector of the tobacco squalene synthase gene is constructed and transformed into tobacco to obtain transgenic tobacco with reduced sterol content. ...

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Abstract

The invention discloses a tobacco squalene synthase?protein, the?amino acid sequence?of which is as shown in SEQIDNO: 1. Tobacco squalene synthase?gene (i) NtSQS (/i) is a?key regulatory gene?in tobacco sterol synthesis pathway?and has a specific high expression level in?tender?tissue.?Virus mediated?gene silencing?vector TRV-NtSQS which is constructed is?inoculated to?Ben's tobacco?so as to obtain a plant with high efficiency of silence. By detection of?phytosterol?content in?Ben's tobacco, the phytosterol?content in the?gene silencing?plant is found to be?observably?decreased?by 41.26%. The above?phenomenon?indicates that?the sterol content?in a plant can be significantly reduced by silencing?the gene. Tobacco squalene synthase?gene (i) NtSQS (/i) is the?key regulatory genein tobacco sterol synthesis pathway, can be used to adjust the sterol content?in tobacco and contributes to?genetic engineering breeding?of low sterol?high quality tobacco.

Description

technical field [0001] The invention relates to tobacco sterol synthesis and regulation key genes, in particular to tobacco squalene synthase gene QUR Application in reducing tobacco sterol content. Background technique [0002] Tobacco is a plant of the genus Nicotiana in the family Solanaceae of the dicotyledonous plant class Picoflowers. There are more than 60 species of Nicotiana. Among the two cultivars, common tobacco (also known as Nicotiana tabacum) occupies the main area, and Nicotiana rustica occupies a small area. Cultivated tobacco can be divided into six types: flue-cured tobacco, sun-cured tobacco, air-dried tobacco, burley tobacco, oriental tobacco and yellow flower tobacco according to the quality characteristics of tobacco leaves, biological characteristics and cultivation methods, among which flue-cured tobacco is the most widely cultivated common tobacco. my country's flue-cured tobacco planting area and total output both rank first in the world. As a l...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N15/60C12N15/82A01H5/00
CPCC12N9/1085C12N15/8243C12Y205/01021
Inventor 陈千思周会娜刘萍萍王燃翟妞金立锋魏春阳陈霞李锋杨军郑庆霞林福呈
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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