Colloidal gold test strip for quick diagnosis of total swine HEV (Hepatitis E Virus) antibody
A rapid diagnosis, colloidal gold technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as inability to detect hepatitis E virus, and achieve the effect of preventing and controlling the prevalence of HEV disease, simple diagnosis and high sensitivity.
Active Publication Date: 2014-05-28
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
[0008] The purpose of the embodiments of the present invention is to provide a colloidal gold rapid diagnostic test strip for pig HEV total antibody and its preparation method, aiming to solve the problem that the existing kits for hepatitis E virus can only specifically detect human hepatitis E virus, but cannot Problems in detecting hepatitis E virus of porcine origin
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preparation example Construction
[0068] like figure 2 Shown, the preparation method of the pig HEV total antibody colloidal gold rapid diagnostic test strip of the embodiment of the present invention comprises the following steps:
[0069] S101: preparing HEV coating antigen;
[0070] S102: Prepare HEV labeled antigen;
[0071] S103: preparing colloidal gold;
[0072] S104: Preparation of HEV antigen-colloidal gold marker;
[0073] S105: Assemble the colloidal gold rapid diagnostic test strip.
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Abstract
The invention discloses a colloidal gold test strip for quick diagnosis of a total swine HEV (Hepatitis E Virus) antibody, and a preparation method of the colloidal gold test strip. The colloidal gold test strip comprises a water-absorbing glass fiber layer, a cellulose nitrate membrane layer, a water-absorbing filter paper layer and a white plastic backing plate. The preparation method comprises the following steps: preparing an HEV coating antigen, an HEV labeled antigen and an HEV labeled antigen-colloidal gold, and assembling the colloidal-gold quick diagnosis test strip. The test strip can be used for detecting the total swine hepatitis E virus antibody, is simple in diagnosis operation and short in detection time and portable, provides a corresponding research basis and technical guarantee for large-scale survey of the HEV eqidemiology, adopts a double antigen sandwich method principle to enable the coating antigen and an antibody in blood serum to generate specific binding and the antigen and antibody reaction to be regenerated by combining with an antigen-antibody complex and a labeled antigen so as to allow the two antigens to bind different antigen binding sites, and is good in specificity and high in sensitivity.
Description
technical field [0001] The invention belongs to the technical field of detection of hepatitis E virus, and in particular relates to a colloidal gold rapid diagnostic test strip for swine HEV total antibody and a preparation method thereof. Background technique [0002] Hepatitis E (Hepatitis E) is a zoonotic infectious disease caused by Hepatitis E virus (HEV) transmitted through the digestive tract. The disease is prone to develop into fulminant hepatitis in adolescents, can lead to miscarriage in pregnant women, and the case fatality rate is as high as 21%. HEV is widely distributed and seriously harmful. [0003] Various countries in the world have successively reported pig-derived HEV. The results of comparison with human HEV sequences revealed that the two have extremely high homology. Later, it was found that the extracted viral RNA can infect chimpanzees and rhesus monkeys. Subsequently, successive experimental results showed that porcine hepatitis E is a zoonotic d...
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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/536G01N33/56983G01N33/5767
Inventor 兰喜杨彬常晓依柳纪省张韵殷相平李宝玉李学瑞李志勇方玉萍
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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