Recombinant virus expressing VP2 gene of bluetongue virus type 16 and construction method and application of recombinant virus

A bluetongue virus and recombinant virus technology, applied in the biological field, can solve problems such as bluetongue type 16 recombinant vaccines that have not yet been carried out, and achieve the effects of good genetic stability, wide host range and enhanced expression

Active Publication Date: 2019-08-13
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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Problems solved by technology

At present, commercial vaccines include attenuated vaccines and inactivated vaccines, and research on bluetongue 16 recombinant vaccines has not yet been carried out at home and abroad

Method used

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  • Recombinant virus expressing VP2 gene of bluetongue virus type 16 and construction method and application of recombinant virus
  • Recombinant virus expressing VP2 gene of bluetongue virus type 16 and construction method and application of recombinant virus
  • Recombinant virus expressing VP2 gene of bluetongue virus type 16 and construction method and application of recombinant virus

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Embodiment Construction

[0022] The following will be further described in conjunction with the accompanying drawings and examples. Unless otherwise specified, the present invention may adopt conventional techniques in the art. Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.

[0023] The recombinant virus expressing the VP2 gene of type 16 bluetongue virus according to an embodiment of the present invention comprises a virus vector and a shuttle plasmid with the VP2 gene of type 16 bluetongue virus packaged in the virus vector, and the type 16 bluetongue virus VP2 The sequence of the gene is shown in SEQ ID NO.1.

[0024] Preferably, the viral vector is a vaccinia virus vector, and the shuttle plasmid is a vaccinia virus shuttle plasmid pSTKE.

[0025] Preferably, the upstream of the VP2 gene of type 16 bluetongue virus also has a kozak sequence.

[0026] The ...

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Abstract

The invention relates to a recombinant virus expressing a VP2 gene of a bluetongue virus type 16 and a construction method and application of the recombinant virus. The recombinant virus includes a virus vector and a recombinant shuttle plasmid which has the VP2 gene of the bluetongue virus type 16 and is packaged in the virus vector, and the sequence of the VP2 gene of the bluetongue virus type 16 is shown in SEQ ID NO.1. Accordingly, the recombinant virus rVTT-VP2 expressing the VP2 gene of the bluetongue virus type 16 (BTV-16) is constructed and screened for the first time at home and abroad, and identified through a molecular biological method. By means of ten times of repeated plaque purification and 20 times of continuous passage, PCR identification results show that the VP2 gene isintegrated into a genome of the recombinant vaccinia virus, and a TK gene cannot be amplified; it is indicated that the recombinant vaccinia virus rVTT-VP2 genetic stability is good, and the virus ispurified.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a recombinant virus expressing the VP2 gene of type 16 bluetongue virus and its construction method and application. Background technique [0002] Bluetongue virus (BTV) is a member of the Orbivirus genus in the Reoviridae family. Its infection mainly relies on arthropods such as Culicoides and sand flies. Bluetongue is an animal infectious disease that must be reported by the World Organization for Animal Health (Office International Des Epizooties, OIE). After my country joined the World Trade Organization, it was also stipulated as a first-class animal disease. The major harm of bluetongue disease is mainly reflected in the high fatality rate of sheep and goats, which causes economic losses of more than 300 million U.S. dollars every year, and consumes huge manpower and material resources. As of 2019, there are 27 serotypes of bluetongue disease, and at least 7 serotypes of BT...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/863C12N15/46A61K39/15A61P31/14C12R1/93
CPCC12N7/00C12N15/86C07K14/005A61K39/12A61P31/14C12N2710/24021C12N2710/24052C12N2710/24043C12N2720/12122C12N2720/12134A61K2039/5256A61K2039/552
Inventor 金宁一李昌张克龙许汪杜寿文田明尧鲁会军李霄郝鹏飞宋利娜姜宇航
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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