Natural circulating microbial agent for preventing plant diseases and insect pests and preparation method thereof
A technology of microbial agent and natural circulation, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc.
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[0028] A natural circulation microbial agent for preventing plant diseases and insect pests, quality standard:
[0029]
[0030] According to experiments, the bacterial strains involved in the present invention are all known bacterial strains, and the bacterial agents using the bacterial beads all have the same water purification effect.
[0031] The preparation method of the natural circulation microbial bacterial agent for preventing plant diseases and insect pests, the steps are as follows:
[0032] (1) Production method of culture medium
[0033] The production method of this culture medium is as follows, every 1L of water is added 0.5g potassium dihydrogen phosphate (KH 2 PO 4 ), 0.2g magnesium sulfate heptahydrate (MgSO 4 7H 2 O), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH 4 CI), 0.05g calcium chloride dihydrate (CaCI 2 2H 2 O), 0.3g yeast extract, 1ml mineral solution (each 1L contains 25% (v / v) hydrogen chloride (HCl) 1ml, zinc chloride (ZnCI ...
experiment example 1
[0036] Experimental Example 1: Effect
[0037]
[0038]Experiments were conducted on spinach, cabbage and radish in Weifang Vegetable Planting Base for 6 months from April to September 2013. The experimental group was diluted 100-fold with water and sprayed (spinach and cabbage) or root irrigation (radish) on the above-ground parts of the plants every week. The control group was not administered this formulation. Compared with the control group, the diseases of the spinach group and the radish group were significantly reduced, the resistance was enhanced, and the yield increased by about 18% (weight); the growth of the cabbage group was better.
[0039] Detection benchmark
[0040] (1) Take 1L of the medium mentioned above, add 1-3g of agar (agar), stir evenly, autoclave it, and put it in a petridish.
[0041] (2) Evenly spray the 100-fold or 1000-fold diluted natural circulation microbial agent in the solidified culture dish.
[0042] (3) Use a 36w three-wavelength...
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