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Human bladder cancer cells capable of realizing multi-organ metastasis

A technology for bladder cancer cells and bladder cancer, applied in the fields of biology and oncology, can solve the problems of inconsistent characteristics and genetic backgrounds, complex genetic backgrounds, and unclear classification of cancer cell invasion degrees.

Active Publication Date: 2014-07-09
许传亮 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] As far as the research field of bladder cancer is concerned, although bladder cancer cell lines have been established at present, due to their early establishment, their genetic background is complex, their passage status is unstable and their tumorigenicity is poor, and their characteristics and genetic background are also inconsistent. , resulting in uneven or even non-expression of bladder cancer-specific markers, and the existing cell lines are not clear about the degree of invasion of cancer cells, so the currently commonly used bladder cancer cell lines can no longer meet the requirements of modern scientific research

Method used

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  • Human bladder cancer cells capable of realizing multi-organ metastasis
  • Human bladder cancer cells capable of realizing multi-organ metastasis
  • Human bladder cancer cells capable of realizing multi-organ metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The establishment of embodiment 1CH-2 cell line

[0099] (a) Source:

[0100] Tumor stage: T2-T4

[0101] Chief complaint: hematuria

[0102] Cystoscopy: Bladder trigone 2×2cm space occupying

[0103] Pathology: high-grade urothelial carcinoma

[0104] Surgery: bladder cancer resection

[0105] (b) Primary cell culture:

[0106] Take 0.5 × 0.5 cm of cancer tissue for primary cell culture. For the method, please refer to the general method.

[0107] The fresh bladder cancer tissue digested with collagenase can grow adherently about 5 hours after centrifugation and plating. A large number of cell clones can appear in about 5 days, and mesenchymal cells and epithelial cells can be seen mixed growth at this time. The mesenchymal cells can be basically cleared after three passages by differential digestion.

[0108] (c) Animal model establishment:

[0109] The cells were inoculated into immunodeficient mice. For the first time, the CH-2 cell line was planted in 3 fe...

Embodiment 2

[0110] Example 2 Biological characteristics of bladder cancer cell line CH-2 with multi-organ metastasis

[0111] Morphological observation: the cell line is spindle-shaped or polygonal ( figure 1 ), the cell shape is stable.

[0112] Proliferation activity: CCK-8 reagent was used to detect the proliferation activity of CH-2 cell line, and it was found that its doubling interval was 48-72 hours ( figure 2 ).

[0113] Passage activity: The cell line has been passed to the 86th passage, the cell shape and growth rate remain unchanged, and the recovery is good after cryopreservation.

[0114] Expression of related proteins and expression of markers in multi-organ metastatic bladder cancer:

[0115] a. Immunohistochemical method with mouse anti-human keratin antibody CK AE1 Detect the source and species of mouse tumor in situ. The results showed that patients with urothelial carcinoma of the urinary tract highly expressed CK AE1 protein, but no expression in interstitial ti...

Embodiment 3

[0116] Example 3 Study on the transfer rate and tumor formation rate of the low-invasive bladder cancer cell line CH-2

[0117] The cells were inoculated into immunodeficient mice. For the first time, the CH-2 cell line was planted in 40 female nude mice, and 38 nude mice were successfully tumor-bearing, and all of the mice had metastasis to lymph nodes or other organs. The above experiment was repeated three times, and the average tumor-bearing success rate and metastasis rate were taken. It was found that the tumor-bearing success rate of the modified cell line was about 75-80%, and the metastasis rate was 100%.

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Abstract

The invention provides human bladder cancer cells capable of realizing multi-organ metastasis, and more specifically relates to a novel multi-organ metastasis bladder cancer cell line CH-2 which is preserved in China Center for Type Culture Collection (CCTCC) on August, 26th, 2013. Preservation number of the novel multi-organ metastasis bladder cancer cell line CH-2 is CCTCC No.C2013129. Cellular morphology and serial subcultivation characteristics of the novel multi-organ metastasis bladder cancer cell line CH-2 are stable; tumor forming performance is excellent; metastasis performance is high; the novel multi-organ metastasis bladder cancer cell line CH-2 can be used for generating high aggressive bladder cancer cells capable of realizing multi-organ metastasis, can be combined with low aggressive bladder cancer cell lines, and is used for selecting medicines used for treating bladder cancer with different characteristics, and selecting genes relating to metastasis.

Description

technical field [0001] The invention belongs to the fields of biology and oncology, and in particular, the invention relates to the establishment and application of a novel multi-organ metastasis bladder cancer cell. Background technique [0002] The occurrence and development of various human diseases are very complicated, and in-depth exploration of the pathogenesis and therapeutic mechanism of various diseases cannot be carried out on patients. However, it is possible to follow the Animal Protection Law, conduct research on various diseases and life phenomena through animals, and then deduce it to human beings to explore the mysteries of human life and the laws of the occurrence and development of human diseases, so as to control the occurrence and development of human diseases, cure diseases and Reduce the suffering of human beings, improve the quality of life, and prolong the life of human beings. [0003] For a long time, people have found that it is difficult to prom...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027A61K49/00C12Q1/02C12Q1/68
Inventor 许传亮徐伟东任善成韦荣超吴承耀孙颖浩
Owner 许传亮
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