Bidirectional regulating therapeutic vaccine for gliomas and preparation method thereof

A glioma and therapeutic vaccine technology, applied in the field of multivalent therapeutic vaccines, can solve problems such as toxic side effects and uncertain efficacy, and achieve the effects of low cost, improved average survival rate, and enhanced anti-tumor immune response

Inactive Publication Date: 2014-07-23
中国人民解放军南京军区福州总医院四七六医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chemotherapy drugs are limited to the blood-brain barrier and drug side effects, the efficacy is not sure yet

Method used

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  • Bidirectional regulating therapeutic vaccine for gliomas and preparation method thereof
  • Bidirectional regulating therapeutic vaccine for gliomas and preparation method thereof
  • Bidirectional regulating therapeutic vaccine for gliomas and preparation method thereof

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Embodiment Construction

[0028] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0029] 1. Preparation of bidirectionally regulated therapeutic vaccine for glioma

[0030] 1. FAT10 154-162 Epitope peptide, FAT10 155-163 Epitope peptide, FAT10 49-57 Design and synthesis of epitope peptides

[0031] FAT10 designed by the present invention 154-162 Epitope peptide, FAT10 155-163 Epitope peptide, FAT10 49-57 The primary structure of the epitope peptide is as figure 1 As shown, each epitope is connected with the transmembrane sequence or the endoplasmic reticulum retention signal s...

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Abstract

The invention discloses a bidirectional regulating therapeutic vaccine for gliomas and a preparation method thereof. The vaccine is a compound of FAT10 multivalent epitope peptide and a Tim-3 genetic recombinant expression vector, wherein the FAT10 multivalent epitope peptide is composed of epitope FAT10154-162 / FAT10155-163 / FAT1049-57 and a trans-membrane sequence HIV-Tat40-48, an endoplasmic reticulum retention signal sequence DELK and a joint sequence; the trans-membrane sequence is located at an amino terminal; the endoplasmic reticulum retention signal sequence is at a carboxyl terminal; the epitope is connected to the trans-membrane sequence or the endoplasmic reticulum retention signal sequence through the joint sequence. The vaccine can successfully enter cells and target endoplasmic reticulum, and effectively promote the epitope FAT10154-162 / FAT10155-163 / FAT1049-57 to enter an MHC-1 (major histocompability complex-1) antigen presentation pathway to excite specific CTL (cytotoxic T lymphocyte) response; meanwhile, the Tim-3 plays roles in inhibiting immune escape and assisting to simulate T cell activation, so as to generate a wide immune activation effect and to induce strong antitumor immunity.

Description

technical field [0001] The invention relates to a multivalent therapeutic vaccine, in particular to a bidirectional regulation therapeutic vaccine for neuroglioma and a preparation method thereof. Background technique [0002] Glioma is a tumor derived from the neuroepithelium. It is the most common intracranial malignant tumor, accounting for about 40%-50% of all intracranial tumors. Due to its high incidence, high recurrence rate, and high mortality rate, it has become a major tumor therapy. problem. Traditional treatment methods are mainly surgery, radiotherapy and chemotherapy to improve the quality of life of patients. However, total resection of gliomas located in important functional areas is difficult. Radiation therapy is the routine treatment for almost all types of glioma, but the efficacy evaluation is different. Except for medulloblastoma, which is highly sensitive to radiotherapy, and ependymoma, which is moderately sensitive, other types are not sensitive to...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K48/00A61P35/00
Inventor 袁邦清杨曌沈汉超吴贤群陈羽建林立
Owner 中国人民解放军南京军区福州总医院四七六医院
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