Preparation method of near-infrared response nanocage and application of near-infrared response nanocage in tumor immune combined therapy

A nanocage and near-infrared technology, applied in the medical field, can solve the problems of limiting the clinical benefits of patients, and achieve the effects of uniform and controllable mesopore size, good biological safety, large specific surface area and pore volume

Active Publication Date: 2021-06-01
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are a large number of clinical (pre) studies of this combination regimen, but the combination therapy is only effective in some patients, which seriously limits the clinical benefits of patients

Method used

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  • Preparation method of near-infrared response nanocage and application of near-infrared response nanocage in tumor immune combined therapy
  • Preparation method of near-infrared response nanocage and application of near-infrared response nanocage in tumor immune combined therapy
  • Preparation method of near-infrared response nanocage and application of near-infrared response nanocage in tumor immune combined therapy

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0063] This embodiment provides a method for preparing a near-infrared responsive nanocage, comprising the following steps:

[0064] (1) prepare gold nanocage (AuNC), the method is as follows:

[0065] (S1) 500 μL of Ag nanocubes with a concentration of 3 nmol / L was added to 5 mL of polyvinylpyrrolidone (PVP) ionic water solution with a concentration of 1 mg / mL, and then heated and boiled for 10 min with constant stirring to obtain a mixture a;

[0066] (S2) Take 0.5mmol HAuCl 4 The aqueous solution was added to mixture a at a rate of 45mL / h, and the system was continuously refluxed for 30min until the color became stable, and then cooled to room temperature to obtain mixture b;

[0067] (S3) Centrifuge the mixture b, remove AgCl with saturated NaCl solution, and then wash with deionized water to remove residual PVP and NaCl to obtain AuNC.

[0068] (II) Preparation of mesoporous silica-coated gold nanocages (AuNC@mSiO 2 ),Methods as below:

[0069] (SS1) Add 4 mL of AuNC ob...

Embodiment 2

[0080] This example provides a method for preparing a near-infrared responsive nanocage, the difference from Example 1 is that in step (S1), the heating and boiling time is 15 minutes; in step (S2), HAuCl 4 The addition rate of the aqueous solution is 50mL / h, and the time for continuous reflux is 40min; in the step (SS1), the reaction is continuously stirred at room temperature for 14h; in the step (SS2), the centrifugation is performed at 7000g for 20min; The washing is alternately washed 4 times with ethanol and deionized water; the stirring reaction is carried out at 55°C for 12h; in step (SSS2), the heating reaction is carried out at 90°C for 8h; the centrifugation is carried out at 7000g Centrifuge for 15 minutes; in step (SSS3), the heating is 75°C, and the reaction time is 5h; in step (SSSS2), the heating is 45°C, and the reaction time is 28h.

[0081] Other operations and raw material consumption are all the same as in Example 1.

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PUM

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Abstract

The invention relates to a preparation method of a near-infrared response nanocage and application of the near-infrared response nanocage in tumor immune combined therapy. The preparation method of the near-infrared response nanocage comprises the four steps of preparing AuNC, preparing AuNC-coated mSiO2, preparing ASP and preparing an ASP intersection V. The near-infrared response nanocage has the advantages of good biocompatibility, safety, near-infrared region photothermal conversion, high drug loading capacity and the like. The drug-loaded nanocage can effectively deliver drugs into tumor cells and convert light energy into heat energy, light heat and a BRAF inhibitor are combined to inhibit tumor growth, induce immunogenic necrosis of the tumor cells, promote release of tumor-related antigens and generate systematic anti-tumor immune response, and the drug-loaded nanocage cooperates with an anti-PD-1 antibody to inhibit tumor growth. According to the gold nanocage, MAPK molecular targeting and anti-PD-1 immune combined treatment is realized, and a new strategy is provided for tumor treatment.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a preparation method of a near-infrared responsive nanocage and its application in combined tumor immunotherapy. Background technique [0002] In recent years, with the deepening of the research on the mechanism of tumor immune escape, immunotherapy, especially the immune detection point targeting programmed cell death 1 (PD-1) and its ligand (programmed cell death-ligand1, PD-L1) Inhibitors significantly prolong patient survival in a variety of tumors. However, PD-1 / PD-L1 immunotherapy still has a serious clinical bottleneck problem, that is, the response rate of monotherapy is only 10%-40%. The reason is that PD-1 / PD-L1 immune checkpoint blockade depends on CD8 in the tumor microenvironment + T cells, while most patients lack immune cells in the tumor microenvironment, that is, immune "cold" tumors. In order to solve this problem, a large number of explorations in combina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/437A61K47/69A61P35/00B22F1/00B22F1/02B22F9/24B82Y5/00B82Y40/00
CPCA61K41/0052A61K41/0042A61K31/437A61K47/6949A61P35/00B82Y5/00B22F9/24B82Y40/00B22F1/0553B22F1/054B22F1/16A61K2300/00
Inventor 刘小伟
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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