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Extraction method of Radix Ophiopogonis medicinal material

An extraction method and technology of Ophiopogon japonicus extract are applied in the field of extracting Ophiopogon japonicus and Ophiopogon japonicus medicinal materials, which can solve the problems such as the decrease of the yield of the Ophiopogon japonicus extract, and achieve obvious anti-myocardial ischemia and meet the requirements of the yield. Effect

Inactive Publication Date: 2014-08-27
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after alcohol precipitation, part of the polysaccharide components of Ophiopogon japonicus are precipitated, resulting in a decrease in the yield of Ophiopogon japonicus extract, while most of the fructose is retained

Method used

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  • Extraction method of Radix Ophiopogonis medicinal material
  • Extraction method of Radix Ophiopogonis medicinal material

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0032] Comparison experiment of fructose content, extract yield and total sugar content of extract at different soaking time:

[0033] Method: Weigh about 7g of Ophiopogon japonicus, add 100ml of water and heat to 90±1℃, keep it for 0-8 hours, heat to boiling, reflux and extract for 1h; add 100ml of water for the second and third time, directly heat to boiling, and extract separately 1h, 0.5h, combine the extracts, take 2ml of the extracts on the SPE column, wash with water and dilute to 10ml, use HPLC-ELSD to detect the fructose content, and concentrate the extracts under reduced pressure to 1ml equivalent to 0.75g of medicinal materials, and carry out 75% alcohol Precipitate; filter after standing, concentrate the filtrate under reduced pressure to 1ml equivalent to 1.6g medicinal materials, and carry out 85% alcohol precipitation; filter after standing, adjust the pH of the filtrate to 9 with NaOH solution; filter after standing, adjust the pH of the filtrate to 6.5 with dilute...

Embodiment 1

[0038] Weigh about 72g of Ophiopogon japonicus, add 1000ml of water and heat to 90±1℃, keep soaking for 8 hours, heat to boiling, reflux and extract for 1 hour; filter out the extract, extract Ophiopogon japonicus for the second and third times, add them separately 1000ml of water was directly heated to boiling, refluxed for 1h, 0.5h, the extract was filtered, and the three extracts were combined; the fructose content of Ophiopogon japonicus in the extract was measured to be 21.0%.

[0039] The extract is concentrated under reduced pressure to 1ml equivalent to 0.75g of medicinal materials and subjected to 75% alcohol precipitation; after placing it is filtered, and the filtrate is concentrated under reduced pressure to 1ml equivalent to 1.6g of medicinal materials, subjected to 85% alcohol precipitation; after being placed, it is filtered and the filtrate is adjusted with NaOH solution The pH is 9; after being placed, it is filtered, the filtrate is adjusted to pH 6.5-7.0 with di...

Embodiment 2

[0041] Weigh about 70g of Ophiopogon japonicus, add 350ml of water and heat to 61±1℃. After soaking for 0.5 hours, heat to boiling, reflux and extract for 1 hour; filter out the extract, and extract Ophiopogon japonicus for the second and third time. 350ml of water is directly heated to boiling, refluxed for 1h, 0.5h, the extract is filtered, and the three extracts are combined; the fructose content of the Ophiopogon japonicus in the extract is determined to be 6.2%.

[0042] The extract is concentrated under reduced pressure to 1ml equivalent to 0.75g of medicinal materials and subjected to 75% alcohol precipitation; after placing it is filtered, and the filtrate is concentrated under reduced pressure to 1ml equivalent to 1.6g of medicinal materials, subjected to 85% alcohol precipitation; after being placed, it is filtered and the filtrate is adjusted with NaOH solution The pH is 9; after standing, it is filtered, the filtrate is adjusted to pH 6.5-7.0 with dilute hydrochloric a...

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Abstract

The invention discloses an extraction method of a Radix Ophiopogonis medicinal material. The method comprises the following steps: 1, immersing the Radix Ophiopogonis medicinal material in hot water, heating after the immersion until boiling, and carrying out reflux extraction; 2, concentrating the above obtained extract liquid, carrying out alcohol precipitation, and filtering; and 3, adjusting the pH value of the above obtained filtrate, and carrying out reduced pressure drying to obtain a Radix Ophiopogonis extract. The method can greatly improve the content of fructose in the Radix Ophiopogonis extract.

Description

Technical field: [0001] The invention relates to a traditional Chinese medicine extraction method, in particular to an extraction method of Ophiopogon japonicus medicinal materials. The fructose content of the Ophiopogon japonicus extract obtained by the method is greatly increased. Background technique: [0002] Ophiopogon japonicus is a commonly used traditional Chinese medicine, derived from the dried roots of the Liliaceae plant Ophiopogon japonicus. It has the functions of nourishing yin, nourishing body fluid, nourishing the lungs and clearing the heart. Modern pharmacological research results show that Ophiopogon japonicus can promote humoral immunity, regulate nucleic acid metabolism, improve animal hypoxia tolerance and improve coronary circulation. Its injection has good effects in treating coronary heart disease, myocardial infarction, and angina pectoris. These physiological and pharmacological effects of Ophiopogon japonicus are related to the sugar contained in it. ...

Claims

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Application Information

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IPC IPC(8): A61K36/8968A61P9/10A61K125/00
Inventor 刘岩王冀李伟徐波杨峰王卫国
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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