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Aspergillus versicolor strain with antibacterial activity

A technology of Aspergillus versicolor and antibacterial activity, which is applied in the field of Aspergillus versicolor, can solve the problems of separation of antibacterial active substances, repeated discovery of increased natural antibacterial active products, etc., and achieve good development and application value and strong inhibitory effect

Inactive Publication Date: 2014-08-27
HEILONGJIANG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] Isolation of new antimicrobial active substances from microorganisms of common environmental origin is increasingly difficult due to the increasing chance of reproducible natural antimicrobial active products by traditional research methods

Method used

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  • Aspergillus versicolor strain with antibacterial activity
  • Aspergillus versicolor strain with antibacterial activity
  • Aspergillus versicolor strain with antibacterial activity

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specific Embodiment approach 1

[0013] Specific embodiment one: the Aspergillus versicolor with bacteriostatic activity in this embodiment is Aspergillus versicolor (Aspergillus versicolor) CWJ2, preserved in China Center for Typical Culture Collection (CCTCC), preservation address is Wuhan University, Wuhan City, preservation date is April 2014 On March 16, the deposit number is CCTCC NO: M2014130.

[0014] After the present embodiment Aspergillus versicolor CWJ2 is cultivated on PDA solid medium for 5 days, texture is velvety; Tool a large amount of conidia structure, green, edge white (such as figure 1 ); no exudate; mild musty smell; the opposite side of the colony is yellowish to orange in varying degrees. The top of the conidiophores of Aspergillus versicolor CWJ2 swelled into an apical capsule, which was spherical (such as figure 2 ). The surface of the top capsule is covered with radial stalks.

[0015] In this embodiment, Aspergillus versicolor CWJ2 can grow on PDA medium, and the optimum growth...

specific Embodiment approach 2

[0016] Specific embodiment 2: In this embodiment, Aspergillus versicolor CWJ2 is screened from healthy Acanthopanax plants in Maoershan area, Heilongjiang Province, China. Screening is carried out according to the following steps: select healthy Acanthopanax roots, rinse them with tap water, blot dry with sterile filter paper, cut them into 1cm segments, and carry out surface disinfection according to the following aseptic procedures: alcohol with a volume concentration of 75% Rinse for 1 min—10% hydrogen peroxide rinse for 15 min—75% alcohol rinse for 1 min—sterile water rinse for 3 times. Place the sterilized experimental material in PDA solid medium, and culture it in a constant temperature incubator at 28°C for 10 to 15 days. After mycelium grows around the experimental material, pick the mycelium and transfer it to a plate (PDA solid medium) Separation and purification were performed by streaking multiple times, and the purified strain was placed in a slant medium (PDA so...

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Abstract

The invention relates to an Aspergillus versicolor strain, particularly an Aspergillus versicolor strain with antibacterial activity. The invention provides an Aspergillus versicolor strain, which has favorable inhibiting actions on Bacillus subtilis, Bacillus pumilus, Staphylococcus aureus and other bacteria and provides references for developing new antibacterial drugs and food preservatives. The Aspergillus versicolor with antibacterial activity is Aspergillus versicolor CWJ2, and is collected at China Center for Type Culture Collection; and the collection date is April 16th, 2014, and the collection number is CCTCC NO:M2014130. The Aspergillus versicolor CWJ2 has strong inhibiting actions on Bacillus subtilis, Bacillus pumilus, Staphylococcus aureus and other bacteria.

Description

technical field [0001] The invention relates to a strain of Aspergillus versicolor. Background technique [0002] As an important class of microbial resources, plant endophytes have become a research hotspot. They are widely distributed in plant tissues and organs and are microbial flora with extremely metabolic diversity. Since the scientist Fleming discovered the first antibiotic—penicillin, the discovery and application of antibiotics have successfully prevented and controlled the diseases and infections caused by microorganisms. However, in recent years, with the wide application of broad-spectrum antibiotics such as penicillins and cephalosporins, various antibiotics are constantly updated, and there are irrational use of antibiotics in clinical practice, which leads to the continuous enhancement of microbial resistance and limits the emergence of drug-resistant strains. The clinical effects of existing antibiotics have been compromised. To solve this problem, the most...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61P31/04A01P1/00A23L3/3571C12R1/66
Inventor 郑春英郭晓璐崔宇徐慧超
Owner HEILONGJIANG UNIV
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