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Ion chromatography on-line pretreatment for measuring glyphosate in genetically modified soybean oil

A technology of transgenic soybean oil and ion chromatography, which is applied in the field of on-line pretreatment determination of glyphosate in transgenic soybean oil by ion chromatography column switching method, to achieve the effect of reducing manpower consumption, saving complicated operation process and saving time

Inactive Publication Date: 2014-09-10
ZHEJIANG UNIV
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Problems solved by technology

Many previous studies have shown that very low concentrations of glyphosate can promote the growth of cancer cells and have a strong carcinogenic effect

Method used

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  • Ion chromatography on-line pretreatment for measuring glyphosate in genetically modified soybean oil
  • Ion chromatography on-line pretreatment for measuring glyphosate in genetically modified soybean oil

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Embodiment Construction

[0023] The invention discloses an online pretreatment method for determining glyphosate in transgenic soybean oil by switching ion chromatographic columns. The system includes a sample injector 1, a six-way valve 2, a reverse phase column 3, a ten-way valve 5, and an enrichment column 4. Anion separation column 6, four-source gradient pump 7, infusion pump 9, conductivity detector 10, waste liquid bottle 13, sampler 1 is connected to six-way valve 2, and six-way valve 2 is connected to four-source gradient pump 7 and The reverse phase column 3 is connected, the enrichment column 4 is connected to the ten-way valve 5, the ten-way valve 5 is respectively connected with the infusion pump 9, and the anion separation column 6, and the anion separation column 6 is sequentially connected with a suppressor and a conductivity detector 10 And waste bottle 13.

[0024] The ion chromatography column switching method is an on-line pretreatment method for the determination of glyphosate in ...

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Abstract

The invention discloses ion chromatography on-line pretreatment for measuring glyphosate in genetically modified soybean oil. The pretreatment comprises the following steps: firstly, mixing a certain volume of a sample to be measured with water according to a certain ratio, performing ultrasonic extraction, dissolving glyphosate in the sample in water, subsequently removing water-soluble organic matrix on line by using a high performance liquid chromatography reversed-phase column, retaining useless ions by a followed enriching column, after column switching, separating the ions which are eluted by using leacheate in an anion chromatographic column, and detecting by using an inhibition conductivity method. The application of an on-line pretreatment system is significant for removing water-soluble organisms in a genetically modified soybean oil sample and measuring the content of pesticide glyphosate in the soybean oil. Due to adoption of the method, the state that a disposable C18 column is adopted to remove the organism in the conventional pretreatment is changed, labor consumption is reduced, and the sample pretreatment time and the complex operation process are saved.

Description

technical field [0001] The invention relates to an ion chromatographic column switching system, in particular to a new method for determining glyphosate in transgenic soybean oil by using an ion chromatographic column switching method for on-line pretreatment. Background technique [0002] Genetically modified food is a food that transfers certain biological genes to other species or uses genetically modified organisms as raw materials. In order to meet the different needs of people, transgenic technology changes the nutritional quality and shape of target species, and improves the consumption quality of biological products by modifying the genetic material of organisms. The superiority of genetically modified foods and their possible safety hazards and side effects have received widespread attention in recent years, and aroused a lot of thinking. [0003] Glyphosate-resistant transgenic soybeans are the crop with the largest planting area and the largest yield among all tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
Inventor 贺婕朱岩
Owner ZHEJIANG UNIV
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