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Noctuid granulosis virus, and preparation method and application of preparation containing virus

A technology of granular virus and noctuid, which is applied in the biological field, can solve the problems of pathogens being easily affected by light, such as drug efficacy period, loss of control effect, and short shelf life, so as to reduce the occurrence base of noctuid offspring, prolong the prevention and control time, The effect of improving quality

Active Publication Date: 2014-09-24
镇江市润宇生物科技开发有限公司 +1
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  • Claims
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Problems solved by technology

The disadvantages of existing biological preparations are that the host is narrow, the disease is slow, the effect is poor, the pathogen is easily affected by light and the efficacy period is short, and the preparation is not resistant to high temperature storage above 40 degrees, and the shelf life will lose the efficacy

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  • Noctuid granulosis virus, and preparation method and application of preparation containing virus
  • Noctuid granulosis virus, and preparation method and application of preparation containing virus
  • Noctuid granulosis virus, and preparation method and application of preparation containing virus

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Embodiment

[0017] The source of the Spodoptera granulosa virus GXW9-3 of the present invention is the corpse of the sick Spodoptera litura found and collected by the inventor on August 13, 2004 (high temperature and strong light season) on wild eggplants in the south of Zhenjiang. And on September 20, 2013, it was preserved in Wuhan University, China (China Center for Type Culture Collection), and its preservation number is CCTCC NO:V201340.

[0018] In this example, preparations containing Spodoptera granulosa virus GXW9-3 were prepared by the following method:

[0019] (1) The highly pathogenic Spodoptera granulosomal virus GXW9-3 isolated and purified from the corpses of the larvae of the diseased Spodoptera moth was freeze-dried at -5°C, -15°C, and -75°C for use. Due to the difference in moisture content of the material before freezing, the freezing time of each temperature is 80-100 minutes, 1700-1800 minutes, 1200-1300 minutes respectively.

[0020] (2) Breeding in the wild and in...

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Abstract

The invention relates to a virus, and a preparation method and application of a preparation containing the virus, particularly a noctuid granulosis virus, and a preparation method and application of a preparation containing the virus, belonging to the technical field of biology. After the noctuid granulosis virus GXW9-3 is inoculated into larva food of senile noctuid and the like, the larvae can have cytopathy after eating the food; and the carcases collected at 24th-32th hours are disinfected with 75% alcohol on the body surface, pulverized and made into the high-content granulosis virus GXW9-3 preparation. The preparation can be prepared into required concentrations according to the characteristics of occurrence and hazards of the Noctuidae pests on different plants to control the noctuid larvae with great hazards, high overeating property and high chemical resistance. The preparation has the advantages of favorable control effect and long control time, and can effectively lower the noctuid descendant occurrence base number. The preparation has the functions of controlling the outbreak and hazards of the noctuid pests, promoting the ecological equilibrium, protecting the plant growth, enhancing the yield and improving the quality.

Description

technical field [0001] The invention relates to a virus, a preparation method and application of a preparation containing the virus, in particular to a spodoptera granulosa virus, a preparation method and application of a preparation containing the virus, and belongs to the field of biotechnology. Background technique [0002] For more than half a century, the use of chemical pesticides to control pests has made great contributions to increasing agricultural production and income. However, problems such as environmental and agricultural product pollution, ecological imbalance, and food safety caused by long-term dependence and extensive use of chemical pesticides are also increasingly apparent. It is imperative to reduce the use of highly toxic and high-residue pesticides, and to promote pollution-free production or control technologies, and the biological control of pests is particularly urgent. More than 200 species of viruses parasitic on agricultural pests have been fou...

Claims

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Application Information

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IPC IPC(8): C12N7/00A01N63/00A61P7/04C12R1/93
Inventor 高小文
Owner 镇江市润宇生物科技开发有限公司
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