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Sheep semen dilution preserving fluid and preparation method thereof

A technology for preserving liquid and semen, applied in the field of animal reproduction, can solve problems such as reduction and unsatisfactory acrosome integrity rate, and achieve the effects of simple production method, slowing sperm death, and reducing disease infection and spread.

Inactive Publication Date: 2014-10-01
赤峰市农牧科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are a large number of sheep frozen semen diluents and diluted preservation solutions on the market; fresh semen dilutions and diluted preservation solutions are less, and their main components are mostly glucose, sodium citrate, and antibiotics. The sperm viability and plasma membrane integrity The acrosome integrity rate is not ideal, and after 48 hours, there is a relatively large decrease, see Table 1

Method used

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  • Sheep semen dilution preserving fluid and preparation method thereof
  • Sheep semen dilution preserving fluid and preparation method thereof
  • Sheep semen dilution preserving fluid and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Sodium citrate 1.4g; fructose 2.0g; glucose 0.55g; D-galactose 0.45g; L-lysine 1.25g; fresh egg yolk centrifugal supernatant 15ml; sodium selenite 5.0mg; Sterilized double distilled water 100.0ml.

Embodiment 2

[0032] Sodium citrate 1.2g; fructose 1.8g; glucose 0.35g; D-galactose 0.25g; L-lysine 1.0g; fresh egg yolk centrifugal supernatant 12ml; sodium selenite 3.0mg; Sterilized double distilled water 100.0ml.

Embodiment 3

[0034] Sodium citrate 2.0g; fructose 2.5g; glucose 0.6g; D-galactose 0.5g; L-lysine 1.3g; fresh yolk centrifugal supernatant 20ml; sodium selenite 6.0mg; α-tocopherol 180IU; Sterilized double distilled water 100.0ml.

[0035] The sodium citrate described in the three embodiments is sodium citrate dihydrate, and its molecular formula is C 6 h 5 Na 3 o 7 2H 2 O.

[0036] combine figure 1 It can be seen that the specific production method of embodiment 1 is:

[0037] Make double distilled water first, measure 100.0ml of double distilled water, and autoclave at 110°C for 10 minutes to obtain sterilized double distilled water A. Accurately weigh 1.4g of sodium citrate, 0.55g of glucose, 0.45g of D-galactose, and 1.25g of L-lysine, respectively add them to sterilized double distilled water A, heat in a water bath at 50-60°C, and carry out sequential Stir clockwise to dissolve for 5-10 minutes, then autoclave at 110°C for 10 minutes to obtain solution B. Aseptically draw fre...

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Abstract

The invention discloses a sheep semen dilution preserving fluid and a preparation method thereof. The sheep semen dilution preserving fluid comprises the following raw materials: 1.2 to 2.0 g of sodium citrate, 1.8 to 2.5 g of laevulose, 0.3 to 0.6 g of glucose, 0.2 to 0.5 g of D-galactose, 1.0 to 1.3 g of L-lysine, 10 to 20 ml of fresh yelk centrifugal supernatant, 2.5 to 6.0 mg of sodium selenite, 75 to 180 IU of alpha-tocopherol, and 100.0 ml of sterilized double distilled water. The sheep semen dilution preserving fluid comprises three types of saccharides, so as to better provide energy for sperms and prolong the survival time of the sperms. Due to the adoption of L-lysine, sperm death can be slowed down, sperm athletic capability can be enhanced, and sperm motility can be improved. Due to the adoption of sodium selenite and Alpha-tocopherol, the sheep semen dilution preserving fluid is higher in biological oxidation resistant function; plasma membranes of the sperms can be protected from being injured by peroxides, so that the sperm structure can be stabilized, and the sperm function can be preserved.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a semen dilution preservation solution for improving the quality and fertilization rate of fresh goat semen after long-distance transportation and a preparation method thereof. Background technique [0002] There are two main types of semen used in artificial insemination of sheep, namely fresh semen and frozen semen. The former is often referred to as fresh semen, and the latter is referred to as frozen semen. In order to improve the utilization rate of breeding rams and facilitate the storage and transportation of semen, some breeding farms and livestock improvement workstations often produce frozen semen for sheep breeding work. However, the equipment for producing frozen semen is expensive, liquid nitrogen needs to be replenished in time during storage and transportation, the technical requirements for semen thawing are relatively high, and the fertili...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 韩天龙王敏毛冉李清泉石岩王丽萍李志明
Owner 赤峰市农牧科学研究院
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