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Synthetic method of mycorrhizal seedlings of crusty puffball

A hard-skinned puffball and a synthetic method are applied in the fields of botanical equipment and methods, horticulture, and application, which can solve the problems of low mycorrhizal infection rate and difficulty in artificially synthesizing mycorrhizal seedlings, and achieve the effect of reducing the number of times.

Active Publication Date: 2016-01-13
贵州宏臻菌业投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to artificially synthesize mycorrhizal seedlings at present, or the rate of mycorrhizal infection is very low during artificial synthesis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take by weighing the various components of the preparation mother seed medium in the following proportions: 100 g of potato, 10 g of glucose, 1 g of ammonium tartrate, 2 g of peptone, MgSO 4 1g, KH 2 PO 4 1g, 15g agar, 500ml water; mix the components, adjust the pH to 4.0 to obtain the mother culture medium, and place the mother culture medium in a 25mm×200mm test tube;

[0035] (2) Collect wild moderately mature fruiting bodies of hard-coated puffballs, operate aseptically in an ultra-clean workbench, use a scalpel to cut out a tissue block of 2 mm × 5 mm × 10 mm in the middle part of the fruiting body, and put the tissue block into a container containing female In the test tube of the seed medium, the test tube is placed in the environment of 27 ° C ± 2 ° C, and cultured in the dark, until the mycelium is covered with the inclined surface of the test tube, the hard skin puffball female species is obtained;

[0036] (3) Take by weighing the various components of ...

Embodiment 2

[0044] (1) Take by weighing the various components of the preparation mother seed medium in the following proportions: 200g of potato, 20g of glucose, 2g of ammonium tartrate, 4g of peptone, MgSO 4 2g, KH 2 PO 4 2g, 20g agar, 1500ml water; mix the components, adjust the pH to 6.0 to obtain the mother culture medium, and place the mother culture medium in a test tube of 25mm×200mm;

[0045] (2) Collect wild moderately mature fruiting bodies of hard-coated puffballs, operate aseptically in an ultra-clean workbench, use a scalpel to cut out a tissue block of 2 mm × 5 mm × 10 mm in the middle part of the fruiting body, and put the tissue block into a container containing female In the test tube of the seed medium, the test tube is placed in the environment of 27 ° C ± 2 ° C, and cultured in the dark, until the mycelium is covered with the inclined surface of the test tube, the hard skin puffball female species is obtained;

[0046] (3) Take by weighing the various components of ...

Embodiment 3

[0055] (1) Take by weighing the various components of the preparation mother seed medium in the following proportions: 140g of potato, 14g of glucose, 1g of ammonium tartrate, 2g of peptone, MgSO 4 1g, KH 2 PO 4 1g, 15g agar, 800ml water; mix the components, adjust the pH to 4.5 to obtain the mother culture medium, and place the mother culture medium in a 25mm×200mm test tube;

[0056] (2) Collect wild moderately mature fruiting bodies of hard-coated puffballs, operate aseptically in an ultra-clean workbench, use a scalpel to cut out a tissue block of 2 mm × 5 mm × 10 mm in the middle part of the fruiting body, and put the tissue block into a container containing female In the test tube of the seed medium, the test tube is placed in the environment of 27 ° C ± 2 ° C, and cultured in the dark, until the mycelium is covered with the inclined surface of the test tube, the hard skin puffball female species is obtained;

[0057] (3) Take by weighing the various components of the ...

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PUM

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Abstract

The invention relates to the field of manual cultivation of fungi, in particular to a sclerodermatales mycorrhiza seedling synthetic method. The sclerodermatales mycorrhiza seedling synthetic method includes the following steps that firstly, an organization containing a sclerodermatales sporocarp is arranged in a mother strain culture medium for cultivation, a sclerodermatales mother strain is obtained, the sclerodermatales mother strain is inoculated into a liquid strain culture medium, a sclerodermatales liquid strain is obtained through cultivation, and / or sclerodermatales spore powder is used for preparing sclerodermatales spore suspension liquid; secondly, an aseptic seedling of a host tree is cultivated; thirdly, when a lateral root is grown from the aseptic seedling, the sclerodermatales liquid strain or the sclerodermatales spore suspension liquid is inoculated at the root system of the aseptic seedling, a matrix is kept wet, the temperature is maintained to be 25+ / -3 DEG C, and a sclerodermatales mycorrhiza seedling is obtained through cultivation. According to the sclerodermatales seedling synthetic method, the symbiotic relationship of the sclerodermatales strain and the host tree can be effectively established, the sclerodermatales mycorrhiza seedling can be successfully synthesized, and the mycorrhiza infection rate can reach up to 85-90 percent.

Description

technical field [0001] The invention relates to the field of artificial cultivation of fungi, in particular to a method for synthesizing mycorrhizal seedlings of crusty puffballs. Background technique [0002] Puffball is an edible and medicinal fungus, which was first recorded in "Famous Doctors". It tastes pungent and flat, and enters the lung meridian. It has the effects of reducing swelling, stopping bleeding, and detoxifying. It is used for sore throat and various bleeding. Modern medical research shows that hard puffballs have various pharmacological effects and clinical applications, and contain steroidal compounds, terpenoids, small molecule nitrogen-containing compounds, puffball polysaccharides, proteins, peptides and other chemical components that are antibacterial and anti-inflammatory , cough, insecticide, anti-tumor cells, has a high medicinal value. Cui Lei reported in 2006 that a new protein was isolated from fresh fruiting bodies of Puffball, which showed a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A01G1/00
Inventor 纪光燕杨君巧杨太茂郭细龙谢飞郭雪玲张文仙
Owner 贵州宏臻菌业投资发展有限公司
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