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Culture medium and method for synthesizing phlebopus portentosus cavity insect galls by using culture medium

A technology for culturing substrate and bolete, which can be applied in the direction of culture medium, planting substrate, horticultural methods, etc., and can solve the problems of slow synthesis and low infection rate.

Pending Publication Date: 2021-06-22
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, the synthetic technology of the boletus fungal cavity gall reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] Weighing 5kg, pearlite 8kg, red soil rock, 7kg, cow dung 1.5kg, rice bran, mix well, smashed into small particles having a particle diameter of 15 mm, 105 ° C vapor sterilization 1.5 h, to obtain a sterile culture matrix. The sterile cultured matrix was mounted into a glass bottle with 75% alcohol sterilized bottom, which was obtained by a glass bottle loaded with a culture matrix. The sterile sputum seeded seeds obtained by tissue culture were transplanted into a glass bottle loaded with a culture matrix. Under the darkness of 4 ° C, 10 days of culture, 10D, and the seeds were seeded, and bovine bacteria were vaccinated in the side root site. The amount of genus and the amount of bovine bacillus were 20g / strain, and the amount of porceae was circa 60 / strain. After inoculation, continued to cultivate at 29 ° C and dark conditions. After 2 months, it was observed that the infection rate of the insects in the sidewalk root boletus cavity was 86.3%. Afte...

Example Embodiment

[0039] Example 2

[0040] 3kg, pearl rock 10kg, red soil, 8kg, cow dung, 1kg, rice bran, mix well, pulverized to small particles having a particle diameter of 10 mm, 110 ° C Steam Sterilization 2H, to obtain a sterile culture matrix. The sterile cultured matrix was mounted into a glass bottle with 75% alcohol sterilized bottom, which was obtained by a glass bottle loaded with a culture matrix. The sterile vine sprout seeded obtained by tissue culture is transplanted into a glass bottle loaded with a culture matrix. Under the dark conditions, at 28 ° C culture 15D to the vine sprout seed, the bovine bacteria is vaccinated in the side root site. The amount of the bullococcus species is 10 g / strain in a mixed genus, and the amount of the mixed genus is 50 / strain. After inoculation, continued to cultivate at 28 ° C, dark conditions. After 2 months, it was observed that the infection rate of the insects in the sidewalk roots of the vineenae is 84.9%, and after 3 months, it was foun...

Example Embodiment

[0041] Example 3

[0042] It is called 4kg, pearlite 9kg, red soil, 0.5kg, rice bran, 5kg of rice bran, mix well, smashed into small particles having a particle size of 8 mm, 115 ° C steam sterilization 1.5 h, to obtain a sterile culture matrix. The sterile cultured matrix was mounted into a glass bottle with 75% alcohol sterilized bottom, which was obtained by a glass bottle loaded with a culture matrix. The sterile vine sprocket seeds obtained by tissue culture were transplanted into a glass bottle loaded with a culture matrix. Under the darkness of the darkness, at 27 ° C culture 20D to the vine seeds, the bovine bacteria were vaccinated in the side root site. The amount of genus and the mixture of bovine bacillus is 30 g / strain, and the amount of the mixed germ is 40 / strain. After inoculation, continued to cultivate at 27 ° C, dark conditions. After 2 months, it was observed that the infection rate of the worms in the sidewalk root bovia cavity was 89.6%. After 3 months, i...

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Abstract

The invention relates to the technical field of fungus bionic cultivation, in particular to a culture medium and a method for synthesizing phlebopus portentosus cavity insect galls by using the culture medium. The culture medium is prepared from the components: 3-5 parts of wood chips, 8-10 parts of perlite, 6-8 parts of red loam, 0.5-1.5 parts of cow dung and 4-6 parts of rice bran. When the culture medium is utilized and the method for synthesizing the phlebopus portentosus cavity insect galls is used for culturing the phlebopus portentosus cavity insect galls, the infection rate of the phlebopus portentosus cavity insect galls can reach 80% or above after the phlebopus portentosus cavity insect galls are cultured for 2 months and can reach 90% or above after 3 months.

Description

technical field [0001] The invention relates to the technical field of biomimetic cultivation of fungi, in particular to a culture substrate and a method for synthesizing Dictyostelium boletus fungal cavity galls using the culture substrate. Background technique [0002] Phlebopus portentosus belongs to the family Boletinellaceae, and Phlebopus belongs to the genus Phlebopus. It is distributed abroad in tropical regions such as Thailand, Sri Lanka, Vietnam, Indonesia, Brazil, Mexico, Australia, New Zealand (Maria 2007, Bandala 2004, Watling 2001, Barbara1987, Peglerl986, Heinemann 1982). Domestically, it is mainly distributed in Yunnan, and also in Guangxi and Hainan (Zang Mu et al. 1986, 2006; Yang Zhuliang and Zang Mu 2003). Boletus dulcis is rich in protein, crude fat, total sugar, crude fiber, various mineral elements and amino acids, and is a kind of edible fungus with high nutritional value (R.Sanmee2003, Zhang Chunxia et al. Delicious edible fungi loved by consumers...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A01G22/40A01G24/23A01G24/15A01G24/20A01G24/12A01G24/25A01G7/06
CPCA01G7/06A01G18/00A01G18/20A01G22/40A01G24/12A01G24/15A01G24/20A01G24/23A01G24/25
Inventor 刘静杨天伟张春霞何明霞王文兵许欣景高锋方艺伟
Owner YUNNAN INST OF TROPICAL CROPS
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