Effective method for extracting proteins from Radix Chuanmingshinis Violacei residues

A technology of protein and ginseng, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as less research, and achieve the effects of simple operation, low production cost, and short extraction time

Inactive Publication Date: 2014-12-03
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the 8 kinds of amino acids necessary for the human body account for more than 40% of the total amino acid content. They may be one of the functional components of Chuanming ginseng, but there are few studies on the use of Chuanming ginseng residue to extract protein.

Method used

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  • Effective method for extracting proteins from Radix Chuanmingshinis Violacei residues
  • Effective method for extracting proteins from Radix Chuanmingshinis Violacei residues
  • Effective method for extracting proteins from Radix Chuanmingshinis Violacei residues

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Embodiment 1

[0045] Chuanming ginseng powder, mixed evenly with alkaline solution of pH 12.5 according to the ratio of liquid to material 24:1, ultrasonic power 185W, ultrasonic temperature 34°C, ultrasonic extraction for 31min, centrifugation, second ultrasonic extraction, combined supernatant, and hydrochloric acid Adjust the pH to 2.0, precipitate, centrifuge, take the precipitate, wash 2 times with 95% ethanol, then wash 3 times with ultrapure water, adjust the pH to 7.0, 300pa, freeze-dry at -70°C, use Dechuan Mingshen protein powder, and measure the protein The extraction rate is 43.49%.

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Abstract

The invention discloses an effective method for extracting proteins from Radix Chuanmingshinis Violacei residues. The method comprises the following steps: drying ethanol extracted Radix Chuanmingshinis Violacei residues at 60DEG C to a constant weight, and crushing to Radix Chuanmingshinis Violacei residues; and uniformly dissolving the Radix Chuanmingshinis Violacei residues in an alkali, carrying out ultrasonic extraction, centrifuging, carrying out secondary ultrasonic extraction, mixing the above obtained supernatants, precipitating by using an acid, centrifuging, taking the obtained precipitate, washing by using alcohol, washing by using water, and carrying out vacuum freeze drying to obtain Radix Chuanmingshinis Violacei protein powder.

Description

Technical field [0001] The present invention involves a method of using ultrasonic auxiliary alkali acid sinking method to efficiently extract Kawamopathensis protein from the Kawaida scum. Background technique [0002] Kawaidoshen, also known as Mingyou, Mingsha ginseng, and Turksuyin, is the Umbelliferae plant. chuanminshen violaceum The root of Sheh et Shan is a unique plant -specific plant in China and a site of Sichuan production channels. The distributed area is located in the subtropical humid seasonal climate zone, with an altitude of 200 ~ 1000m, 29 ° ~ 32 ° north latitude, 105 ° east longitude ~112 °. The main producing areas are Sichuan (Qingbei River, Jinsang, Jianyang, Cangxi, Weiyuan, Beichuan, Pingwu, Bazhong, Nanchuan), Hubei (Yichang, Dangyang) and other places.The spleen and other effects, treating fever and injury, lung fever cough, spleen deficiency and food, and weakness after the disease. Chuanming ginseng contains polysaccharides, protein, polypeptides, ami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30C07K1/14
Inventor 秦文董红敏牛小勇刘继沈丽雯何靖柳李路
Owner SICHUAN AGRI UNIV
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