Stable alpha-amylase detection kit

A detection kit and amylase technology, which are applied in the field of medical immunology in vitro diagnosis, can solve the problems that the amount of pigment added is difficult to control, the enzymatic reaction cannot be calculated, and the measured results are quite different, and the measurement results are accurate, reliable and stable. Improved, easy-to-use effects

Active Publication Date: 2014-12-10
上海睿康生物科技有限公司
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Problems solved by technology

There are many kinds of substrates used in the determination of amylase. The early determination method used starch as the substrate. In the 1960s, the determination method using dyed starch as the substrate appeared. The inhomogeneity of the enzymatic reaction can not be calculated according to the chemical formula
When using starch from different sources, manufacturers, and batch numbers as the substrate, the measured results are quite different, and the method is not easy to standardize
The amount of pigment added in dyed starch is not easy to control, the operation is complicated, and it is not suitable for automatic analysis

Method used

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Examples

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Embodiment 1～3

[0028] The present examples 1-3 relate to a stable α-amylase detection kit, which consists of reagent R1 and reagent R2. The components and concentrations of the reagents R1 and R2 are shown in Table 1:

[0029] Table 1

[0030]

[0031]

[0032] The AMY detection kits described in Examples 1 to 3 are applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 2.

[0033] Analysis method: rate method, that is, reagent R1; the amount of R2 is 240ul and 60ul respectively, and the sample volume is 6ul; add 6ul sample to 240ul reagent R1, add 60ulR2 after 5min at 37°C, start reading with a delay of 60 seconds, and the reading time is about 120 seconds; The detection wavelengths are respectively the main wavelength 405nm and the subwavelength 505nm.

[0034] Adopt this reagent and above-mentioned assay method, adopt the curve of the AMY standard substance (from Landau) that ...

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Abstract

The invention provides a stable alpha-amylase detection kit. The stable alpha-amylase detection kit is composed of a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: 0.5-100mM buffer solution, 20-200mM NaCl, 10-100mM CaCl2, 1-10g / L sodium dodecyl benzene sulfonate, 0.1-10KU / L alpha-glucosidase, 0.1-10g / L stabilizer and 0.1-10g / L preservative; the reagent R2 is prepared from the following components: 0.5-100mM buffer solution, 0.5-10g / L 4,6-ethylidene-nitrophenylcarbimide-alpha-D-maltoheptaoside, 1-10g / L sodium dodecyl benzene sulfonate, 0.1-10g / L stabilizer and 0.1-10g / L preservative. The sodium dodecyl benzene sulfonate is added, so that the performances of the kit are improved, the kit has the good repetitiveness, high sensitivity and wide linear range, and reagents are stable.

Description

technical field [0001] The invention relates to the field of medical immune in vitro diagnosis, in particular to a stable α-amylase detection kit. Background technique [0002] Amylase (α-Amylase), referred to as AMS or AMY, is a type of enzyme that can hydrolyze starch molecules. It generally acts on α-1,4-glucans such as soluble starch, amylose, and glycogen to hydrolyze α-1, 4-Glycosidic bonds, resulting in dextran, maltose and glucose molecules. Amylase is abundant in the pancreas and salivary glands. Pancreatic amylase is excreted into the digestive tract in an active state by the pancreas. It is the most important enzyme that hydrolyzes carbohydrates. It belongs to α-amylase like the amylase secreted by salivary glands. Amylase has a small molecular weight and can be filtered through the glomerulus and excreted with urine. When suffering from pancreatic diseases or pancreatic exocrine dysfunction, amylase activity can be increased or decreased. Therefore, the determi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
CPCG01N33/52
Inventor 李伟奇李杰房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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