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Oscillation appliance for improving dissolved oxygen level of microculture process

A technology for microbial cultivation and utensils, applied in the field of microbial cultivation utensils, can solve problems such as narrow application range, low content of metabolic target products, low wet weight of bacteria, poor dissolved oxygen, etc., to increase the contact area and facilitate growth and metabolism , Increase the effect of dissolved oxygen environment

Inactive Publication Date: 2014-12-17
ZHEJIANG SHENGHUA BIOK BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies of the prior art, the present invention provides a vibration appliance with a wide range of applications and is used to increase the dissolved oxygen level in the microbial cultivation process, to solve the problem that the existing vibration appliances have a narrow application range and dissolve oxygen in the microbial cultivation process. Poor oxygen leads to defects in microbial fermentation metabolism target product content and bacterial cell wet weight

Method used

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  • Oscillation appliance for improving dissolved oxygen level of microculture process
  • Oscillation appliance for improving dissolved oxygen level of microculture process
  • Oscillation appliance for improving dissolved oxygen level of microculture process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] attached figure 1 : Oscillating appliance, comprises opening 10, appliance body 20; Opening 10 is positioned at appliance body 20 tops, and opening 10 is a hollow cylinder; Appliance body 20 is made up of appliance body top 21 and appliance body bottom 22; Appliance body top 21 is horizontal The cross-section is a triangular hollow trihedron, and the upper surface of the upper part 21 of the appliance body is partially open, and the opening is sealed and connected to the lower end of the opening 10; the lower part 22 of the appliance body is an inner hollow trihedron whose top is large and bottom is small, and the bottom is sealed by a sealing plane 221 Platform; the hollow trihedron in the upper part 21 of the appliance body is sealed and connected with the hollow triangular platform in the lower part 22 of the appliance body.

Embodiment 2

[0034] attached figure 2 , with Figure 4 : Oscillating appliance, comprises opening 10, appliance body 20; Opening 10 is positioned at appliance body 20 tops, and opening 10 is a hollow cylinder; Appliance body 20 is made up of appliance body top 21 and appliance body bottom 22; Appliance body top 21 is horizontal The cross-section is a square hollow tetrahedron, and the upper surface of the upper part 21 of the appliance body is partially open, and the opening is sealed and connected to the lower end of the opening 10; the lower part 22 of the appliance body is a regular quadrangular inner hollow with a large top and a small bottom, and the bottom is sealed by a sealing plane 221 Platform; the hollow tetrahedron in the upper part 21 of the appliance body is sealed and connected with the hollow square platform in the lower part 22 of the appliance body; the upper part of the oscillating appliance is provided with an inclined hollow branch pipe 211, and the branch pipe is fix...

Embodiment 3

[0036] attached image 3 : Oscillating appliance, comprises opening 10, appliance body 20; Opening 10 is positioned at appliance body 20 tops, and opening 10 is a hollow cylinder; Appliance body 20 is made up of appliance body top 21 and appliance body bottom 22; Appliance body top 21 is horizontal The cross-section is an octagonal hollow octahedron, and the upper surface of the upper part 21 of the appliance body is partially open, and the opening is sealed and connected with the lower end of the opening 10; Octagonal platform; the hollow octahedron in the upper part 21 of the appliance body is sealed and connected with the hollow octagonal platform in the lower part 22 of the appliance body.

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Abstract

The invention relates to a microculture appliance, in particular to an oscillation appliance for improving the dissolved oxygen level of a microculture process. The oscillation appliance comprises an opening portion 10 and an appliance body 20, the opening portion 10 is located above the appliance body 20, the opening portion is a hollow body A, the appliance body 20 is composed of an appliance body upper portion 21 and an appliance body lower portion 22, the appliance body upper portion 21 is a hollow body B with an included angle on the inner side, an opening is designed in a surface portion of the upper end of the appliance body upper portion 21, the opening is in sealed connection with the lower end of the opening portion 10, the appliance body lower portion 22 is a big-end-up inner hollow cavity with an included angle on the inner side, the bottom of the big-end-up inner hollow cavity is sealed by a sealing plane 221, and the hollow body B of the appliance body upper portion 21 is in sealed connection with the inner hollow cavity of the appliance body lower portion 22. The oscillation appliance for improving the dissolved oxygen level of the microculture process has the advantages that the application range is wide, the dissolved oxygen level of the microculture process can be improved, and thereby, microbial metabolism target product content and thallus wet weight can be increased.

Description

technical field [0001] The invention relates to a microorganism cultivating appliance, in particular to an oscillating appliance used for increasing the dissolved oxygen level in the microorganism cultivating process. Background technique [0002] Microorganisms are widely used in agriculture, industry, medicine, food, environmental protection and many other fields. In the research process of microorganisms, it is usually necessary to culture them in shake flasks, and then carry out further fermentation cultures in fermentors or other bioreactors. The growth state of microorganisms in the shake flask is directly related to the fermentation production level of microorganisms in the fermenter, and an important indicator to determine the growth state of microorganisms is the dissolved oxygen level. [0003] There are usually three types of shake flasks currently used for microbial culture: (1) round-bottomed triangular conical flasks with smooth inner walls, and the medium in ...

Claims

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Application Information

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IPC IPC(8): C12M1/24
CPCC12M23/08C12M27/16
Inventor 吴黎诚徐顺清魏华洪丹丹熊莉孙俊杰
Owner ZHEJIANG SHENGHUA BIOK BIOLOGY
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