Molecular markers and their application of major QTLs regulating grain length in maize

A molecular marker, corn technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of inconsistent results in grain length QTL analysis, and achieve the effect of simple identification method, high selection efficiency, and clear selection target.

Active Publication Date: 2016-09-14
JIANGSU ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Due to the limitations of the type of mapping population (mostly F2:3 families), the precision of the genetic linkage map used (mostly ~200SSR markers), and the evaluation method of grain length (depth), there is no consistent result for the QTL analysis of grain length.

Method used

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  • Molecular markers and their application of major QTLs regulating grain length in maize

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Experimental program
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Embodiment Construction

[0017] The experimental methods in the following examples are conventional experimental methods unless otherwise specified. The experimental reagents and consumables described in the following examples are from General Biochemical Reagent Company unless otherwise specified.

[0018] In this embodiment, the detailed steps for obtaining the main QTL molecular markers for regulating the grain length of corn are as follows:

[0019] (1) Construction of maize triple testcross (TTC) population and measurement of grain length

[0020] 82 IBM recombinant inbred line populations with high genetic exchange rates of different genotypes were crossed with their parents B73, Mo17 and F1 respectively to obtain TC(B73), TC(Mo17) and TC(F1) triple testcross populations. 82 IBM populations of different genotypes, TC(B73), TC(Mo17) and TC(F1) triple testcross populations were planted in the experimental base according to random grouping. Each genotype material was planted in a single row, with...

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Abstract

The invention relates to the field of crop molecular breeding, and specifically discloses a molecular marker for regulating the main effect QTL of corn grain length and an application thereof. Molecular markers for the main QTL regulating grain length in maize consisted of two pairs of primers, InDel14 and umc1269. A method for assisting the selection of long-grain corn, comprising: extracting the genomic DNA of the corn to be tested, performing PCR amplification with the above primers InDel14 and umc1269, and when the amplification products with a length of 236bp and 350bp are obtained, the corn to be tested is a candidate long-grained corn. Kernel maize, using the candidate long-grain maize for breeding. By using the molecular marker of the present invention to assist in the selection of long-grain corn, the length of the grain can be predicted only by detecting the characteristic amplification band of the molecular marker, and the identification method is simple and the selection efficiency is high.

Description

technical field [0001] The invention relates to the field of crop molecular breeding, in particular to a molecular marker for regulating the main effect QTL of corn grain length and its application. Background technique [0002] Corn is an important food crop in the world and has become the second largest crop in my country. With the rapid increase of population and the continuous reduction of arable land, the production of food crops such as corn needs to be greatly increased to meet human needs. The improvement of corn yield is the main technical means to solve the problem of reducing the total amount of grain caused by the reduction of cultivated land. In maize breeding, the improvement of yield per unit area is not only dependent on the increase of planting density, but also the weight of kernels is an important factor affecting yield. [0003] Yield traits are complex quantitative traits controlled by multiple genes. As a direct trait affecting yield, grain traits ca...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N15/113C12Q1/68
Inventor张体付赵涵
OwnerJIANGSU ACAD OF AGRI SCI