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In-vitro cultivation method of gramineous plants

A technology for in vitro culture of gramineous plants, applied in the field of in vitro culture of gramineous plants, can solve the problems of inability to study accurately and short culture time, and achieve the effects of prolonging the time of in vitro culture, improving precision, and avoiding errors

Active Publication Date: 2015-01-14
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

This method was improved by Singh and Jenner (1983), which can realize in vitro culture at room temperature, but the culture time is still relatively short (12-15 days), and it is impossible to accurately study the whole process from the booting stage to the mature stage of wheat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Preparation of culture medium:

[0020] Prepare constant elements according to conventional methods: according to the concentration of 1.66mmol L K 2 SO 4 , 2.5mmol / L KH 2 PO 4 , 0.2mmol / L CaSO 4 2H 2 O and 0.3mmol / L MgSO 4 ·7H 2 O Calculate the required mass of each chemical reagent per liter, and dissolve it in 1 / 2L double-distilled water successively. In order to fully dissolve the reagent, one reagent can be completely dissolved first, and then another reagent can be dissolved for future use;

[0021] Prepare nitrogen nutrient solutions with different concentrations according to conventional methods: take 5 parts of prepared macroelement nutrient solutions, add and dissolve according to the concentration gradient of 0mmol / L, 5mmol / L, 10mmol / L, 15mmol / L, and 20mmol / L respectively NH 4 NO 3 ,spare;

[0022] Prepare trace elements according to conventional methods: according to the concentration of 16.9 mg / L MnSO 4 ·H 2 O, 6.2 mg / L H 3 BO 3 , 0.83 mg / L KI...

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Abstract

The invention relates to an in-vitro cultivation method of gramineous plants. The method is a new method for realizing long-time in-vitro cultivation of gramineous plants from jointing, heading, flowering, filling, milk stage and soft dough stage to ripening. By preparing a nutrient solution containing macroelements, microelements and organic matter which are essential to the growth and development of gramineous plants and through indoor culture, the normal growth and development of gramineous plants in the in-vitro state can be realized to ensure consistent external conditions in the growth and development process of plants, eliminate influence of other factors except to-be-researched factors on the growth and development of plants and improve the accuracy for test and research. The method mainly solves the problem that research on the change of physiological and biochemical indexes in the mature process of gramineous plants cultured in field and the response to certain nutrient elements is easily influenced by numerous external environment factors in the field. The invention can be applied to long-time in-vitro cultivation of all gramineous plants such as wheat, barley, rye, oat, rice and corn, thereby accelerating the breeding of high-yield, high-efficiency and high-quality gramineous crops.

Description

technical field [0001] The invention relates to a method for in vitro cultivation of gramineous plants. Background technique [0002] Traditional plant growth and development research is mainly carried out in the field, and the influencing factors are very complex. The nutrient elements applied to the field are obviously affected by the difference in soil texture and the effect of soil microorganisms, and the absorption and utilization efficiency of nutrients by plants. Therefore, due to the influence of many factors in field planting or pot culture, it is impossible to control the flow of assimilates from the vegetative organs of Gramineae plants to the ears, that is, the relationship between the sink (ear)-source (nutrient elements) cannot be effectively controlled, and the nutrient elements have a great influence on plant growth and development. Research is often imprecise. Therefore, the use of in vitro culture technology can effectively simplify the source-sink relati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周洪华李卫红
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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