Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for tissue culture and rapid propagation of Eucalyptus urophylla dh33-27 variety

A technology of tissue culture rapid propagation and varieties, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of inability to effectively inherit the excellent characteristics of parents, tissue culture work has not been carried out effectively, and excellent characteristics need to be studied. , to achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2016-02-03
广西壮族自治区国有东门林场
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0009] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful propagation of the excellent traits of excellent trees, and the tissue culture and rapid propagation methods that can be effectively popularized and applied. Due to the DH33-27 It is a perennial species of Eucalyptus urophylla. There are few tissue-cultured seedlings in the market and resources are in short supply. However, the tissue culture of this variety has not been effectively carried out. The tissue culture research on the new variety of Eucalyptus urophylla DH33-27 will help the rapid development of this fine variety. It is of great significance to breed, meet the market supply, and improve the ecological and economic benefits of this fine variety

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for tissue culture and rapid propagation of Eucalyptus urophylla dh33-27 variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH33-27 variety, the specific steps are as follows:

[0033] ①Establishment of explants: The selected DH33-27 variety was planted into a test forest. In February, a 4-5-year-old tree with a strong growth, a thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the o...

Embodiment 2

[0041] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH33-27 variety, the specific steps are as follows:

[0042] ①Establishment of explants: The selected DH33-27 variety was planted into a test forest. In February, a 4-5-year-old tree with a strong growth, a thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture rapid propagation method of Eucalyptus urophylla DH33-27 variety, which is realized through the steps of explant establishment, explant disinfection, primary culture, subculture, rooting culture, seedling hardening and transplanting. , A special medium composition was selected for primary culture, subculture and rooting culture. Reasonable seedling hardening measures made Eucalyptus macrophylla grow fast after transplanting, with a high survival rate, and achieved the purpose of high reproduction coefficient and good reproduction effect. Simultaneously, the production cost of the method of the invention is low, and the obtained tissue culture seedlings have consistent genetic traits, which is an effective way for tissue culture and rapid propagation of eucalyptus, and has a wide application range.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus macrocephalus DH33-27 variety. Background technique [0002] Eucalyptus grandis DH33-27 variety (E .urophylla×E . Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 (E ucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G33 (E ucalyptus grandis ) as the male parent hybridization dominant species, which is an excellent eucalyptus variety obtained through asexual propagation. Peeling completely. At the same time, it has excellent comprehensive performance, straight trunk shape, neat forest appearance, small branches, no pests and diseases, large annual average storage volume, obvious economic benefits, and is suitable for large-scale promotion and planting. [0003] Years of popularization tests have proved that ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 陈德文苏勇沈云庞贞武黄全东莫继有吴兵梁秀莉陈允椿韦炳俭唐再生吴满芬俸荣娣邓冬丽付淼吕华丽兰俊石前陈东林李丽芳熊涛王建忠张磊龚卫新
Owner 广西壮族自治区国有东门林场
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com