A method for preparing Cordyceps sinensis strain material using a cultivation room

A technology of Cordyceps sinensis and Cordyceps sinensis, which is applied in the field of preparation of Cordyceps sinensis strain material, which can solve the problems of restriction, low infection activity, limited yield of strain material, etc.

Active Publication Date: 2017-03-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have problems such as low infection activity, low survival rate after injury, or too long time-consuming, or limited yield of strain materials, which is one of the key problems restricting the industrialization of original ecological Cordyceps sinensis medicinal materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Choose an area with convenient transportation and cheap electric power. Use 10m×10m as a room unit to build an alpine plant cultivation room that automatically controls climate conditions. The cultivation room is equipped with 5-layer culture racks for placing plant seedling culture bottles; the installation is conventional in the cultivation room Industrial cold storage uses a central air-conditioning system to adjust the indoor air temperature for cultivation; install spray pipes and central air-conditioning systems to jointly adjust the air humidity in the cultivation room; install a daylight lighting system on the cultivation racks to provide light to cultivated plants; use activated carbon microfiltration membrane air filtration system, Keep the cultivation room dust-free and clean; install ultraviolet lamps in the cultivation room and disinfect regularly. Before entering the cultivation room, the staff should change the disinfected work clothes, shoes, hats, etc. ...

Embodiment 2

[0026] 1. In an area with an altitude of 4500 meters on the Qinghai-Tibet Plateau where railway transportation is convenient, a 20m×20m unit is used to build an alpine plant cultivation room that automatically controls climate conditions. The cultivation room is equipped with a 10-layer culture rack for placing plant seedling culture bottles; the cultivation room Install conventional industrial cold storage with central air-conditioning ventilation system to adjust the indoor air temperature of cultivation; install spray pipes and central air-conditioning system to jointly adjust the indoor air humidity of cultivation; install daylight lighting system on cultivation racks to provide light to cultivated plants; use activated carbon microfiltration membrane air The filter system keeps the cultivation room dust-free and clean; install ultraviolet lamps in the cultivation room and disinfect regularly. Before entering the cultivation room, the staff should change the disinfected work ...

Embodiment 3

[0032] 1. In an industrial zone with complete industrial supporting facilities and convenient material transportation, a unit of length 20m×width 10m is constructed to build an alpine plant cultivation room that automatically controls climate conditions. The cultivation room is equipped with a 3-layer sterilized soil cultivation rack; the cultivation room Install conventional industrial cold storage with central air-conditioning ventilation system to adjust the indoor air temperature of cultivation; install spray pipes and central air-conditioning system to jointly adjust the indoor air humidity of cultivation; install daylight lighting system on cultivation racks to provide light to cultivated plants; use activated carbon microfiltration membrane air The filter system keeps the cultivation room dust-free and clean; install ultraviolet lamps in the cultivation room and disinfect regularly. Before entering the cultivation room, the staff should change the disinfected work clothes,...

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Abstract

The invention relates to a method for preparing a cordyceps sinensis strain material with high infection activity by utilizing a cultivation chamber. According to the method, a psychrophyte tissue culture seedling cultivation chamber of which the climatic conditions can be controlled automatically or manually is established; the climatic factors such as the temperature, the humidity, the illumination and the like of the Qinghai-Tibet Plateau are simulated; psychrophyte tissue culture seedlings are obtained by using a tissue culture rapid propagation and cultivation technology; the prepared cordyceps sinensis resource is sprayed and incubated to the psychrophyte tissue culture seedlings; an adaption environment is created to promote the commensalism of Hirsutella sinensis and psychrophyte tissue during an asexual stage of cordyceps sinensis; the infection rate of the Thitarodes larvae with the cordyceps sinensis material after being subjected to commensalism processing can reach 95.8 to 98.9 percent.

Description

Technical field [0001] The invention relates to a method for preparing cordyceps strain material by using a cultivation room. Background technique [0002] Cordyceps Ophiocordyceps sinensis (Berk.) Sung, Sung, Hywel-Jones & Spatafora is an entomogenous asco fungus that parasitizes the larvae of the Lepidoptera Hepialidae Thitarodes insect, and its anamorph is Hirsutella sinensis Liu, Guo, Yu & Zeng. One generation of Thitarodes, the host of Cordyceps, includes four developmental stages: eggs, larvae, pupae, and adults. The larvae live in underground tunnel-like burrows. They live in underground tunnels. Young and tender rhizomes of alpine plants. [0003] Cordyceps is distributed in high-altitude areas of the Qinghai-Tibet Plateau, its growth range is limited, and its output is extremely limited. It is a rare and nourishing Chinese medicinal material with characteristics of the Qinghai-Tibet Plateau, with important medicinal and economic value. Since there are still many key is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H4/00
Inventor 钟欣张古忍刘昕阚绪甜李少松何健敏
Owner SUN YAT SEN UNIV
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