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Platinum nano-material with activity of four mimic enzymes and preparation method thereof

A technology of platinum nanometer and enzyme imitation, which is applied in the field of nanotechnology and bionics, can solve the problems of difficult purification, high price, harsh operating conditions, etc., and achieve the effect of simple and fast preparation process, small particle size, high dispersibility and stability

Inactive Publication Date: 2015-01-28
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sources of natural enzymes are limited, difficult to purify, and expensive
At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications.

Method used

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  • Platinum nano-material with activity of four mimic enzymes and preparation method thereof
  • Platinum nano-material with activity of four mimic enzymes and preparation method thereof
  • Platinum nano-material with activity of four mimic enzymes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Mix 1 ml of 16 mmol / L chloroplatinic acid aqueous solution with 1 ml of 40 mmol / L trisodium citrate, dilute with 38 ml of double distilled water, and stir for 30 minutes in the dark. Subsequently, 0.2 ml of 50 mmol / L sodium borohydride aqueous solution was added (the addition time was controlled within 2 minutes), the color of the reaction solution changed from light yellow to brownish yellow, and stirring was continued for 1 hour in the dark. The resulting platinum nanomaterial solution had a concentration of 78 mg / L and was stored at 4°C. All glassware used in the above process was soaked in aqua regia, washed thoroughly with double distilled water, and dried.

Embodiment 2

[0037] The solution obtained in Example 1 was drip-coated on a copper grid for detection by a transmission electron microscope. Such as figure 1 As shown, the average particle size of platinum nanoparticles is 2.5 ± 0.4 nm. Such as figure 2 As shown, the interplanar spacing of the obtained nanoparticles is 0.223 nm, which corresponds to the 111 crystal plane of the platinum crystal.

Embodiment 3

[0039] The solution obtained in Example 1 was freeze-dried to obtain a powder, and the obtained powder was subjected to X-ray photoelectron spectroscopy. Such as image 3 As shown, the obtained platinum nanoparticles consist of 86% Pt 0 and 14% Pt 4+ composition.

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Abstract

The invention discloses a platinum nano-material with the activity of four mimic enzymes and a preparation method thereof. The preparation method comprises the following steps: using citrate ions as a modifier, reducing chloroplatinic acid by utilizing sodium borohydride, and preparing the platinum nano-material modified by citric acid. The platinum nano-material is simply and quickly prepared, the particle size is small, and the dispersibility and stability of a water solution are high. The obtained platinum nano-material has the activity of four mimic enzymes at the same time, and comprises peroxidase, oxidase, catalase and superoxide dismutase. The activity of the four mimic enzymes of the platinum nano-material can be adjusted and controlled through the concentration of the citrate ions used in the preparation process.

Description

technical field [0001] The invention relates to a platinum nano material with four simulated enzyme activities (peroxidase, oxidase, catalase, superoxide dismutase) and a preparation method thereof, belonging to the field of nanotechnology and bionic technology. Background technique [0002] Enzymes are very important proteins with catalytic activity in organisms. They have high catalytic efficiency, strong specificity, and mild reaction conditions. The growth, development, reproduction and other life activities of organisms are inseparable from the catalysis of enzymes. However, the sources of natural enzymes are limited, difficult to purify, and expensive. At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications. The artificial nano-enzyme has the characteristics of high c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F1/00B22F9/24B82Y30/00B82Y40/00
Inventor 陈伟何少斌吴钢伟邓豪华彭花萍
Owner FUJIAN MEDICAL UNIV
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