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Syphilis test strip and preparation method thereof, and syphilis detection card and preparation method thereof

A test strip and test card technology, applied in the field of immune detection, can solve the problems of high false positive rate, high sensitivity, interference, etc., and achieve the effect of reducing false positive rate, accurate test results, and realizing detection.

Active Publication Date: 2015-02-04
河南泰宇高分医疗器械有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The advantage of the specific antibody detection method is strong specificity and high sensitivity, but there are two defects. On the one hand, because the detection is IgG antibody, after the human body is infected with Treponema pallidum, it takes more than 2 weeks to produce IgG antibody. Therefore, syphilis cannot be detected in the early stage of infection; on the other hand, syphilis-specific IgG antibodies will be carried for life after syphilis is produced, and even after syphilis treatment, the human body will still carry syphilis-specific IgG antibodies, so simple detection of syphilis-specific IgG antibodies cannot distinguish syphilis from present Whether it is a symptomatic infection or a past infection, the past infection will have some interference on the current infection, and further non-specific antibody detection is needed
[0005] The advantage of non-specific antibody detection method is high sensitivity, but poor specificity and high false positive rate

Method used

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  • Syphilis test strip and preparation method thereof, and syphilis detection card and preparation method thereof
  • Syphilis test strip and preparation method thereof, and syphilis detection card and preparation method thereof
  • Syphilis test strip and preparation method thereof, and syphilis detection card and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0089] The preparation of the cardiolipin-phospholipid binding protein complex of embodiment 1 colloidal gold label

[0090] Dissolve 1g of cardiolipin in 500mL of absolute ethanol, and then dilute to 1L to prepare a 1mg / mL ethanol solution of cardiolipin; dissolve 1g of phospholipid-binding protein in 500mL of water, then dilute to 1L to prepare a 1mg / mL solution aqueous solution of phospholipid-binding protein.

[0091] Mix 100mL cardiolipin ethanol solution with 100mL phospholipid-binding protein aqueous solution, place it at 4℃ for 10h at 37°C for 2h, and obtain cardiolipin-phospholipid-binding protein complex solution; take 50mL5mg / mL colloidal gold solution, and slowly Stir, then slowly add the above cardiolipin-phospholipid-binding protein complex solution, stir at 4°C for 2h, then add 50mL of 1% bovine serum albumin solution, block at 37°C for 1h, centrifuge at 12000rpm for 30min, and use 50mL of 50mM, pH7.4 After reconstitution in phosphate buffer, store at 2-8°C for...

Embodiment 2

[0092] The preparation of the cardiolipin-phospholipid binding protein complex of embodiment 2 colloidal gold label

[0093] Dissolve 0.5g of cardiolipin in 500mL of absolute ethanol, then dilute to 1L, and prepare 0.5mg / mL ethanol solution of cardiolipin; dissolve 5g of phospholipid-binding protein in 500mL of water, and then dilute to 1L, to prepare 5mg / mL of phospholipid-binding protein aqueous solution.

[0094] Take 200mL of cardiolipin ethanol solution and 100mL of phospholipid-binding protein aqueous solution, mix it at 37°C for 1h and then place it at 2°C for 12h to obtain a solution of cardiolipin-phospholipin-binding protein complex; take 50mL of 5mg / mL colloidal gold solution, slowly Stir, then slowly add the above cardiolipin-phospholipid-binding protein complex solution, stir at 2°C for 2h, then add 70mL of 1% bovine serum albumin solution, block at 37°C for 1h, centrifuge at 12000rpm for 30min, and use 50mL of 50mM, pH7.4 After reconstitution in phosphate buffe...

Embodiment 3

[0095] The preparation of the cardiolipin-phospholipid binding protein complex of embodiment 3 colloidal gold labels

[0096] Dissolve 5g of cardiolipin in 500mL of absolute ethanol, and then dilute to 1L to prepare a 5mg / mL ethanol solution of cardiolipin; dissolve 0.5g of phospholipid-binding protein in 500mL of water, and then dilute to 1L to prepare 0.5mg / mL of phospholipid-binding protein aqueous solution.

[0097] Mix 100mL cardiolipin ethanol solution with 200mL phospholipid-binding protein aqueous solution, place at 37°C for 4h, and then place at 8°C for 8h to obtain cardiolipin-phospholipid-binding protein complex solution; take 50mL 5mg / mL colloidal gold solution, and slowly Stir, then slowly add the above cardiolipin-phospholipid-binding protein complex solution, stir at 8°C for 2h, then add 70mL of 1% bovine serum albumin solution, block at 37°C for 1h, centrifuge at 12000rpm for 30min, and use 50mL of 50mM, pH7.4 After reconstitution in phosphate buffer, store a...

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Abstract

The invention relates to the technical field of immunodetection, and particularly relates to a syphilis test strip and a preparation method thereof, and a syphilis detection card and a preparation method thereof. The syphilis test strip comprises a bottom plate (1). The bottom plate (1) is provided with a sample pad (2), a glass cellulose membrane (3), a nitrocellulose membrane (4) and absorbent paper (5) in sequence. The glass cellulose membrane (3) is provided with a colloidal gold-marked treponema pallidum specific antigen and a colloidal gold-marked cardiolipin-phospholipid binding protein complex. A preparation method of the colloidal gold-marked cardiolipin-phospholipid binding protein complex comprises the steps of mixing cardiolipin and phospholipid binding protein; carrying out first incubation to obtain the cardiolipin-phospholipid binding protein complex; mixing the cardiolipin-phospholipid binding protein complex and colloidal gold; carrying out second incubation; and sealing to obtain the colloidal gold-marked cardiolipin-phospholipid binding protein complex. The detection card provided by the invention is simple and convenient to operate, has high sensitivity, high specificity and low false positive rate, and prevents interference of previous infection in current infection.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a syphilis test strip and a preparation method thereof, a syphilis detection card and a preparation method thereof. Background technique [0002] Syphilis is a common sexually transmitted disease that occurs when Treponema pallidum infects the human body. It has been around for hundreds of years and is currently distributed all over the world. It is a very important sexually transmitted disease. According to the source of infection, it is divided into acquired syphilis and congenital syphilis . Among them, acquired syphilis refers to syphilis infected by adults through sexual behavior, blood transfusion, and surgery. It is divided into three stages according to clinical manifestations. The main symptoms include invasion of skin and mucous membranes and involvement of internal organs. It is a relatively serious sexually transmitted disease; Congenital syphilis is the syp...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/532
CPCG01N33/531G01N33/532G01N33/571
Inventor 赵磊席宇
Owner 河南泰宇高分医疗器械有限公司
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