A kind of tissue culture rapid propagation method of Eucalyptus urophylla dh32-28 variety

A technology of tissue culture rapid propagation and varieties, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as inability to effectively inherit the excellent characteristics of parents, tissue culture work has not been effectively carried out, and excellent characteristics need to be studied. Achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2016-02-03
广西八桂种苗高科技集团股份有限公司
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Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0009] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful propagation of the excellent traits of the excellent trees, and the tissue culture and rapid propagation methods that can be effectively popularized and applied. Due to the DH32-28 It is a perennial species of Eucalyptus macrocephalus. There are few tissue culture seedlings in the market and resources are in short supply. However, the tissue culture of this species has not been carried out effectively. The tissue culture research on the new species of Eucalyptus macrocephalus DH32-28 is conducted, and the rapid propagation of this species , to meet the market supply, it is of great significance to improve the ecological and economic benefits of this fine variety

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  • A kind of tissue culture rapid propagation method of Eucalyptus urophylla dh32-28 variety

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Embodiment 1

[0032] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH32-28 variety, the specific steps are as follows:

[0033] ①Establishment of explants: The selected DH32-28 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the obtai...

Embodiment 2

[0041] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH32-28 variety, the specific steps are as follows:

[0042] ①Establishment of explants: The selected DH32-28 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the obtai...

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Abstract

The invention discloses a tissue culture and rapid propagation method of Eucalyptus urophylla DH32-28 variety, which is realized through the steps of explant establishment, explant disinfection, primary culture, subculture, rooting culture, seedling hardening and transplanting. , A special medium composition was selected for primary culture, subculture and rooting culture. Reasonable seedling hardening measures made Eucalyptus macrophylla grow fast after transplanting, with a high survival rate, and achieved the purpose of high reproduction coefficient and good reproduction effect. Simultaneously, the production cost of the method of the invention is low, and the obtained tissue culture seedlings have consistent genetic traits, which is an effective way for tissue culture and rapid propagation of eucalyptus, and has a wide application range.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus urophylla DH32-28 variety. Background technique [0002] Eucalyptus (Eucalyptus) is the general name of Myrtaceae (Myrtaceae) Eucalyptus (Eucalyptus) plants. It is native to Australia. There are more than 100 species, of which more than 40 are widely cultivated. In recent years, all parts of our country are vigorously developing eucalyptus planting, forming a certain scale in Guangdong, Guangxi, Hainan, Sichuan, Yunnan and other provinces, which has produced obvious ecological and economic benefits. [0003] Eucalyptus tail DH32-28 variety ( E. urophylla × E. Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 ( Eucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G46 ( Eucalyptus grandis ) as a hybrid dominant sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 苏勇陈德文陈允椿韦炳俭黄全东沈云庞贞武梁秀莉吴兵吴满芬俸荣娣龚卫新邓冬丽付淼吕华丽唐再生
Owner 广西八桂种苗高科技集团股份有限公司
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