Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dual fluorescence quantitative detection method for fmdv general type and asia 1 type

A general-purpose, fluorescent probe technology, applied in the field of animal disease quarantine and detection, can solve the problems of inability to detect and type foot-and-mouth disease, and achieve good application value, good convenience, and strong specificity

Active Publication Date: 2017-03-01
HENAN CENT FOR ANIMAL DISEASE CONTROL & PREVENTION
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic researchers have established a single serotype RT-PCR diagnostic method for FMD for O, A, and Asia 1 serotypes, they cannot detect and type FMD at the same time, so there is room for further improvement

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dual fluorescence quantitative detection method for fmdv general type and asia 1 type
  • Dual fluorescence quantitative detection method for fmdv general type and asia 1 type
  • Dual fluorescence quantitative detection method for fmdv general type and asia 1 type

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0072] The foot-and-mouth disease virus (FMDV) universal type and Asia 1 type dual fluorescence quantitative detection method provided by the present invention comprises the following steps:

[0073] (1) Primer design

[0074] After a large number of comparisons of the full gene sequences of the general type of foot-and-mouth disease virus and Asia 1 type in GenBank, the highly conserved 2B gene of the general type of foot-and-mouth disease virus and Asia 1 type was selected as a template, and the reverse transcription primer P0 shared by the general type and Asia 1 type was designed , according to the FMDV VP1 conserved gene sequence design general specific primer pair (P1 / P2) and fluorescent probe Probe-P5, according to Asia 1 type specific VP1 gene sequence design specific primer pair (P3 / P4) and fluorescent probe Probe-P6, primers and probe sequences are as follows:

[0075] Primer P0: 5'-cgggaaacgcatgagcagta-3',

[0076] Primer P1: 5'-cggcctctcatccaacaga -3',

[0077] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of animal epidemic disease quarantine and detecting and particularly relates to a double TaqManMGB fluorescent quantitative RT-PCR detecting method for foot and mouth disease virus (FMDV) universal types and Asia 1 types. The method includes primer design, total RNA preparation, reverse transcription, universal type and Asia 1 type standard substance preparation, double FQ-PCR reaction, result judging and the like. By the detecting method which is short in time, fast in response, high in specificity, high in sensitivity, high in throughput, and the like, fast identification and detecting of two viruses can be achieved at the same time by one reaction, the detecting can be completed in 1-2 hours, the requirements for large-batch and fast FMDV identification and detecting can be satisfied, and good application value in the field of FMDV identification and detecting is achieved.

Description

technical field [0001] The invention belongs to the technical field of animal disease quarantine and detection, and specifically relates to a double TaqMan MGB fluorescence quantitative RT-PCR detection method for foot-and-mouth disease virus (FMDV) general type and Asia 1 type. Background technique [0002] Foot and Mouth Disease (FMD) is an acute, febrile and highly contagious disease caused by Foot and Mouth Disease Virus (FMDV) mainly in artiodactyls, which is listed by the World Organization for Animal Health (OIE) as Animal infectious diseases that must be reported are defined as Class I animal diseases in my country. [0003] Foot-and-mouth disease virus can make a variety of animals sick. It mainly affects artiodactyls. Cattle are the most susceptible, followed by pigs, and sheep, goats, camels and other animals. Foot-and-mouth disease spreads quickly and in a wide range. It develops in a sprawling and leaping manner. It is generally endemic and can also form a perio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/686C12Q1/701C12Q2600/112C12Q2563/107C12Q2545/113
Inventor 闫若潜吴志明安春霞张健崔沛谢彩华王淑娟王东方陈涛马震原刘琨刘先敏李茂龙韩庆斌
Owner HENAN CENT FOR ANIMAL DISEASE CONTROL & PREVENTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com