Vaccines and methods to treat lyme disease in dogs

A vaccine composition and immunogenicity technology, which can be used in chemical instruments and methods, vaccines, veterinary vaccines, etc., and can solve the problem of unclear phylotypes of vaccines.

Inactive Publication Date: 2015-02-25
ZOETIS SERVICE LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, more than 20 OspC phylotypes exist, and it is unclear which phylotypes should be included in a vaccine

Method used

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  • Vaccines and methods to treat lyme disease in dogs
  • Vaccines and methods to treat lyme disease in dogs
  • Vaccines and methods to treat lyme disease in dogs

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0095] Example 1. Determination of Borrelia burgdorferi OspC Phylotypes Associated with Lyme Disease in Dogs

[0096] Adult Ixodes scapularis ticks were collected by tagging in southern Rhode Island. The percentage of ticks infected with B. burgdorferi was determined by direct fluorescence microscopy using standard methods and labeled anti-B. burgdorferi antibodies.

[0097] All procedures were performed in accordance with the regulations of the Animal Welfare Act and dogs were maintained according to Farm Canine Husbandry Standard Operating Procedures. Fifteen purpose-bred dogs of both sexes (7 males, 8 females; 9 to 10 weeks of age; Marshall Bioresources) were assigned identification numbers and divided into four study groups, denoted T01(n =4), T02 (n=4), T03 (n=4) and T04 (n=3). Dogs were equipped with Elizabethan collars and housed in apartment-style cages next to each other. Serum was collected from each dog the day before tick infestation. Dogs in study groups T01, ...

example 2

[0113] Example 2. Efficacy of recombinant chimeric Borrelia burgdorferi OspC / OspA vaccine in dogs.

[0114] Thirty dogs, all in good general health, were selected for the study. Blood samples were collected prior to initial vaccination. Dogs received one of the following vaccines, as described in Table 2: T01: PBS (control product); T02: 20 μg / ml OspA+30 μg / ml A12CF (SEQ ID NO: 31); T03: 20 μg / ml mL OspA + 30 μg / mL A10CF (SEQ ID NO: 30). (A12CF consists of epitopes from multiple OspC phylotypes linked together to form a single polypeptide. A10CF also consists of epitopes from multiple OspC phylotypes; its design is similar to that of A12CF.) Dogs in Vaccines were given twice at 8 and 11 weeks of age, and then challenged at 14 weeks of age. Following vaccination, the dogs were observed for 20 minutes for reactions or abnormalities. Observe the swelling, pain, fever, abscess, pus etc. at the injection site on the 1st, 2nd, 3rd and 22nd, 23rd, 24th day. An Elizabeth [E] coll...

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Abstract

The instant invention provides an immunogenic composition comprising an antigenic fragment of OspA protein of Borrelia burgdorferi and a chimeric protein containing antigenic fragments of different phylotypes of OspC protein of Borrelia burgdorferi. Vaccines incorporating the immunogenic composition of the invention, as well as methods of preventing Lyme disease in dogs and/or protecting dogs from Lyme disease using the vaccines are also provided.

Description

technical field [0001] The invention belongs to the field of veterinary medicine. More specifically, the invention is in the field of vaccines for the treatment or prevention of Lyme disease in dogs. Background technique [0002] Lyme disease is a bacterial infection caused by pathogenic spirochetes of the genus Borrelia. Infections can occur in humans, dogs, deer, mice, and other animals, and are transmitted by arthropod vectors, most notably ticks of the genus Ixodes. Borrelia burgdorferi, the most common cause of Lyme disease in North America, was first cultured in 1982. Borrelia are introduced into the host at the site of a tick bite and this site is also the site of the initial characteristic skin lesions (erythema chronicum migrans). In dogs, Lyme disease manifests as arthritis-induced lameness, anorexia, fever, lethargy, lymphadenopathy and, in some cases, fatal glomerular nephritis. Recent studies have shown that the percentage of dogs with active infection in an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02C07K14/20A61K39/00
CPCC07K2319/00A61K2039/552A61K39/0225A61P31/04A61P31/12A61P33/00Y02A50/30
Inventor R.M.洛瑟P.F.M.缪斯J.J.米勒希普Z.许R.T.马科尼C.厄恩哈特
Owner ZOETIS SERVICE LLC
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